Regulated assembly of a supramolecular centrosome scaffold in vitro
Woodruff JB, Wueseke O, Viscardi V, Mahamid J, Ochoa SD, Bunkenborg J, Widlund PO, Pozniakovsky A, Zanin E, Bahmanyar S, Zinke A, Hong SH, Decker M, Baumeister W, Andersen JS, Oegema K, Hyman AA (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 348
Pages Range: 808-812
Journal Issue: 6236
Abstract
The centrosome organizes microtubule arrays within animal cells and comprises two centrioles surrounded by an amorphous protein mass called the pericentriolar material (PCM). Despite the importance of centrosomes as microtubule-organizing centers, the mechanism and regulation of PCM assembly are not well understood. In Caenorhabditis elegans, PCM assembly requires the coiled-coil protein SPD-5.We found that recombinant SPD-5 could polymerize to form micrometer-sized porous networks in vitro. Network assembly was accelerated by two conserved regulators that control PCM assembly in vivo, Polo-like kinase-1 and SPD-2/Cep192. Only the assembled SPD-5 networks, and not unassembled SPD-5 protein, functioned as a scaffold for other PCM proteins. Thus, PCM size and binding capacity emerge from the regulated polymerization of one coiled-coil protein to form a porous network.
Authors with CRIS profile
Additional Organisation(s)
Involved external institutions
University of California, San Diego
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Max-Planck-Institut für Biochemie / Max Planck Institute of Biochemistry
Germany (DE)
University of Southern Denmark / Syddansk Universitet
Denmark (DK)
University of California, Berkeley
United States (USA) (US)
Copenhagen University Hospital
Denmark (DK)
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Max-Planck-Institut für Biochemie / Max Planck Institute of Biochemistry
Germany (DE)
University of Southern Denmark / Syddansk Universitet
Denmark (DK)
University of California, Berkeley
United States (USA) (US)
Copenhagen University Hospital
Denmark (DK)
How to cite
APA:
Woodruff, J.B., Wueseke, O., Viscardi, V., Mahamid, J., Ochoa, S.D., Bunkenborg, J.,... Hyman, A.A. (2015). Regulated assembly of a supramolecular centrosome scaffold in vitro. Science, 348(6236), 808-812. https://dx.doi.org/10.1126/science.aaa3923
MLA:
Woodruff, Jeffrey B., et al. "Regulated assembly of a supramolecular centrosome scaffold in vitro." Science 348.6236 (2015): 808-812.
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