An organic-inorganic hybrid scaffold with honeycomb-like structures enabled by one-step self-assembly-driven electrospinning
Ding Y, Li W, Schubert DW, Boccaccini AR, Roether J, Santos HA (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 124
Article Number: 112079
DOI: 10.1016/j.msec.2021.112079
Abstract
Electrospun organic/inorganic hybrid scaffolds have been appealing in tissue regeneration owing to the integrated physicochemical and biological performances. However, the conventional electrospun scaffolds with non-woven structures usually failed to enable deep cell infiltration due to the densely stacked layers among the fibers. Herein, through self-assembly-driven electrospinning, a polyhydroxybutyrate/poly(ε-caprolactone)/58S sol-gel bioactive glass (PHB/PCL/58S) hybrid scaffold with honeycomb-like structures was prepared by manipulating the solution composition and concentration during a one-step electrospinning process. The mechanisms enabling the formation of self-assembled honeycomb-like structures were investigated through comparative studies using Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) between PHB/PCL/58S and PHB/PCL/sol-gel silica systems. The obtained honeycomb-like structure was built up from nanofibers with an average diameter of 370 nm and showed a bimodal distribution of pores: large polygonal pores up to hundreds of micrometers within the honeycomb-cells and irregular pores among the nanofibers ranging around few micrometers. The cell-materials interactions were further studied by culturing MG-63 osteoblast-like cells for 7 days. Cell viability, cell morphology and cell infiltration were comparatively investigated as well. While cells merely proliferated on the surface of non-woven structures, MG-63 cells showed extensive proliferation and deep infiltration up to 100–200 μm into the honeycomb-like structure. Moreover, the cellular spatial organization was readily regulated by the honeycomb-like pattern as well. Overall, the newly obtained hybrid scaffold may integrate the enhanced osteogenicity originating from the bioactive components, and the improved cell-material interactions brought by the honeycomb-like structure, making the new scaffold a promising candidate for tissue regeneration.
Authors with CRIS profile
Dirk Schubert
Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe)
Aldo Boccaccini
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Judith Roether
Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe)
Involved external institutions
Finland (FI)How to cite
APA:
Ding, Y., Li, W., Schubert, D.W., Boccaccini, A.R., Roether, J., & Santos, H.A. (2021). An organic-inorganic hybrid scaffold with honeycomb-like structures enabled by one-step self-assembly-driven electrospinning. Materials Science and Engineering C, 124. https://dx.doi.org/10.1016/j.msec.2021.112079
MLA:
Ding, Yaping, et al. "An organic-inorganic hybrid scaffold with honeycomb-like structures enabled by one-step self-assembly-driven electrospinning." Materials Science and Engineering C 124 (2021).
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