Dynamic imaging of iel-iec co-cultures allows for quantification of cd103-dependent t cell migration

Enderle K, Dinkel M, Spath EM, Schmid B, Zundler S, Tripal P, Neurath M, Hildner K, Neufert C (2021)

Publication Type: Journal article

Publication year: 2021

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 22

Article Number: 5148

Journal Issue: 10

DOI: 10.3390/ijms22105148

Abstract

Intraepithelial lymphocytes (IEL) are widely distributed within the small intestinal epithelial cell (IEC) layer and represent one of the largest T cell pools of the body. While implicated in the pathogenesis of intestinal inflammation, detailed insight especially into the cellular cross-talk between IELs and IECs is largely missing in part due to lacking methodologies to monitor this interaction. To overcome this shortcoming, we employed and validated a murine IEL-IEC (organoids) ex vivo co-culture model system. Using livecell imaging we established a protocol to visualize and quantify the spatio-temporal migratory behavior of IELs within organoids over time. Applying this methodology, we found that IELs lacking CD103 (i.e., integrin alpha E, ITGAE) surface expression usually functioning as a retention receptor for IELs through binding to E-cadherin (CD324) expressing IECs displayed aberrant mobility and migration patterns. Specifically, CD103 deficiency affected the ability of IELs to migrate and reduced their speed during crawling within organoids. In summary, we report a new technology to monitor and quantitatively assess especially migratory characteristics of IELs communicating with IEC ex vivo. This approach is hence readily applicable to study the effects of targeted therapeutic interventions on IEL-IEC cross-talk.

Authors with CRIS profile

Martin Dinkel Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Eva-Maria Spath Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Benjamin Schmid Optical Imaging Center Erlangen (OICE) Sebastian Zundler Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Philipp Tripal Optical Imaging Center Erlangen (OICE) Markus Neurath Lehrstuhl für Innere Medizin I Kai Hildner Juniorprofessur für Pneumologie/ Immunologie Clemens Neufert Medizinische Fakultät

Additional Organisation(s)

Interdisziplinäres Zentrum für Klinische Forschung

Related research project(s)

A084: The pathogenetic role of tissue-resident memory T cells in acute Graft-versus-Host-disease May 1, 2020 - May 31, 2023

How to cite

APA:

Enderle, K., Dinkel, M., Spath, E.-M., Schmid, B., Zundler, S., Tripal, P.,... Neufert, C. (2021). Dynamic imaging of iel-iec co-cultures allows for quantification of cd103-dependent t cell migration. International Journal of Molecular Sciences, 22(10). https://dx.doi.org/10.3390/ijms22105148

MLA:

Enderle, Karin, et al. "Dynamic imaging of iel-iec co-cultures allows for quantification of cd103-dependent t cell migration." International Journal of Molecular Sciences 22.10 (2021).

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