Impact of zinc-or copper-doped mesoporous bioactive glass nanoparticles on the osteogenic differentiation and matrix formation of mesenchymal stromal cells

Westhauser F, Decker S, Nawaz Q, Rehder F, Wilkesmann S, Moghaddam A, Kunisch E, Boccaccini AR (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Materials MDPI

Book Volume: 14

Article Number: 1864

Journal Issue: 8

DOI: 10.3390/ma14081864

Abstract

Mesoporous bioactive glass nanoparticles (MBGNs) have gained relevance in bone tissue engineering, especially since they can be used as vectors for therapeutically active ions like zinc (Zn) or copper (Cu). In this study, the osteogenic properties of the ionic dissolution products (IDPs) of undoped MBGNs (composition in mol%: 70 SiO2, 30 CaO) and MBGNs doped with 5 mol% of either Zn (5Zn-MBGNs) or Cu (5Cu-MBGNs; compositions in mol%: 70 SiO2, 25 CaO, 5 ZnO/CuO) on human bone marrow-derived mesenchymal stromal cells were evaluated. Extracellular matrix (ECM) formation and calcification were assessed, as well as the IDPs’ influence on viability, cellular osteogenic differentiation and the expression of genes encoding for relevant members of the ECM. The IDPs of undoped MBGNs and 5Zn-MBGNs had a comparable influence on cell viability, while it was enhanced by IDPs of 5Cu-MBGNs compared to the other MBGNs. IDPs of 5Cu-MBGNs had slightly positive effects on ECM formation and calcification. 5Zn-MBGNs provided the most favorable pro-osteogenic properties since they increased not only cellular osteogenic differentiation and ECM-related gene expression but also ECM formation and calcification significantly. Future studies should analyze other relevant properties of MBGNs, such as their impact on angiogenesis.

Authors with CRIS profile

Qaisar Nawaz Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Universitätsklinikum Heidelberg DE Germany (DE) Klinikum Aschaffenburg DE Germany (DE)

How to cite

APA:

Westhauser, F., Decker, S., Nawaz, Q., Rehder, F., Wilkesmann, S., Moghaddam, A.,... Boccaccini, A.R. (2021). Impact of zinc-or copper-doped mesoporous bioactive glass nanoparticles on the osteogenic differentiation and matrix formation of mesenchymal stromal cells. Materials, 14(8). https://dx.doi.org/10.3390/ma14081864

MLA:

Westhauser, Fabian, et al. "Impact of zinc-or copper-doped mesoporous bioactive glass nanoparticles on the osteogenic differentiation and matrix formation of mesenchymal stromal cells." Materials 14.8 (2021).

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