Klein S, Distel L, Neuhuber W (2021)
Publication Type: Journal article
Publication year: 2021
The efficiency of nanoparticle-enhanced radiotherapy was studied by loading MCF-7 and A549 multicellular tumor spheroids (MCTSs) with caffeic acid- and nitrosoniumfunctionalized Au-Fe3O4 nanoheterodimers (Au-Fe3O4 NHDs). Transmission electron microscope images of MCTS crosssectional sections visualized the invasion and distribution of the nitrosonium- and caffeic acid-functionalized Au-Fe3O4 NHDs (NO- and CA-NHDs) in the A549 and MCF-7 MCTSs, whereas the iron content of the MCTSs were quantified using the ferrozine assay. The synergistic impact of intracellular NO- and CA-NHDs and X-ray irradiation on the growth dynamics of the A549 and MCF-7 MCTSs was surveyed by monitoring their temporal evolution under a light microscope over a period of 14 days. The emergence of hypoxia during the spheroid growth was followed by detecting the lactate efflux of MCTSs without and with NO- and CA-NHDs. The performance of the NO- and CA-NHDs as X-ray dose-enhancing agents in the A549 and MCF-7 MCTSs was clarified by performing clonogenic cell survival assays and determining the respective dose-modifying factors for X-ray doses of 0, 2, 4, and 6 Gy. The NO- and CA-NHDs were shown to perform as potent X-ray dose-enhancing agents in A549 and MCF-7 MCTSs. Moreover, the CA-NHDs boosted their radio-sensitizing efficacy by inhibiting the lactate efflux as impairing metabolic reprogramming. A synergistic effect on the MCTS destruction was observed for the combination of both NHDs since the surfactants differ in their antitumor effect.
APA:
Klein, S., Distel, L., & Neuhuber, W. (2021). X-ray dose-enhancing impact of functionalized Au-Fe3O4nanoheterodimers on MCF-7 and A549 multicellular tumor spheroids. ACS Applied Bio Materials. https://dx.doi.org/10.1021/acsabm.0c01494
MLA:
Klein, Stefanie, Luitpold Distel, and Winfried Neuhuber. "X-ray dose-enhancing impact of functionalized Au-Fe3O4nanoheterodimers on MCF-7 and A549 multicellular tumor spheroids." ACS Applied Bio Materials (2021).
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