Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, Brandstätter JH (2021)
Publication Type: Journal article
Publication year: 2021
Book Volume: 35
Pages Range: e21520-
Journal Issue: 5
Bassoon (BSN) is a presynaptic cytomatrix protein ubiquitously present at chemical synapses of the central nervous system, where it regulates synaptic vesicle replenishment and organizes voltage-gated Ca2+ channels. In sensory photoreceptor synapses, BSN additionally plays a decisive role in anchoring the synaptic ribbon, a presynaptic organelle and functional extension of the active zone, to the presynaptic membrane. In this study, we functionally and structurally analyzed two mutant mouse lines with a genetic disruption of Bsn-Bsngt and Bsnko -using electrophysiology and high-resolution microscopy. In both Bsn mutant mouse lines, full-length BSN was abolished, and photoreceptor synaptic function was similarly impaired, yet synapse structure was more severely affected in Bsngt/gt than in Bsnko/ko photoreceptors. The synaptic defects in Bsngt/gt retina coincide with remodeling of the outer retina-rod bipolar and horizontal cell sprouting, formation of ectopic ribbon synaptic sites-and death of cone photoreceptors, processes that did not occur in Bsnko/ko retina. An analysis of Bsngt/ko hybrid mice revealed that the divergent retinal phenotypes of Bsngt/gt and Bsnko/ko mice can be attributed to the expression of the Bsngt allele, which triggers cone photoreceptor death and neurite sprouting in the outer retina. These findings shed new light on the existing Bsn mutant mouse models and might help to understand mechanisms that drive photoreceptor death.
APA:
Ryl, M., Urbasik, A., Gierke, K., Babai, N., Joachimsthaler, A., Feigenspan, A.,... Brandstätter, J.H. (2021). Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes. The FASEB Journal, 35(5), e21520-. https://dx.doi.org/10.1096/fj.202001962R
MLA:
Ryl, Miriam, et al. "Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes." The FASEB Journal 35.5 (2021): e21520-.
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