Structure and adsorption behavior of high hydrostatic pressure-treated β-lactoglobulin

Kieserling H, Giefer P, Uttinger M, Lautenbach V, Nguyen T, Sevenich R, Lübbert C, Rauh C, Peukert W, Fritsching U, Drusch S, Maria Wagemans A (2021)

Publication Language: English

Publication Type: Journal article, Original article

Publication year: 2021

Journal

Book Volume: 596

Pages Range: 173-183

DOI: 10.1016/j.jcis.2021.03.051

Abstract

Hypothesis: High hydrostatic pressure treatment causes structural changes in interfacial-active β-lactoglobulin (β-lg). We hypothesized that the pressure-induced structural changes affect the intra- and intermolecular interactions which determine the interfacial activity of β-lg. The conducted experimental and numerical investigations could contribute to the mechanistic understanding of the adsorption behavior of proteins in food-related emulsions. Experiments: We treated β-lg in water at pH 7 with high hydrostatic pressures up to 600 MPa for 10 min at 20 °C. The secondary structure was characterized with Fourier-transform infrared spectroscopy (FTIR) and circular dichroism (CD), the surface hydrophobicity and charge with fluorescence-spectroscopy and ζ-potential, and the quaternary structure with membrane-osmometry, analytical ultracentrifugation (AUC) and mass spectrometry (MS). Experimental analyses were supported through molecular dynamic (MD) simulations. The adsorption behavior was investigated with pendant drop analysis. Findings: MD simulation revealed a pressure-induced molten globule state of β-lg, confirmed by an unfolding of β-sheets with FTIR, a stabilization of α-helices with CD and loss in tertiary structure induced by an increase in surface hydrophobicity. Membrane-osmometry, AUC and MS indicated the formation of non-covalently linked dimers that migrated slower through the water phase, adsorbed more quickly due to hydrophobic interactions with the oil, and lowered the interfacial tension more strongly than reference β-lg.

Authors with CRIS profile

Involved external institutions

Technische Universität Berlin Germany (DE) Leibniz-Institut für Werkstofforientierte Technologien (IWT) Germany (DE)

How to cite

APA:

Kieserling, H., Giefer, P., Uttinger, M., Lautenbach, V., Nguyen, T., Sevenich, R.,... Maria Wagemans, A. (2021). Structure and adsorption behavior of high hydrostatic pressure-treated β-lactoglobulin. Journal of Colloid and Interface Science, 596, 173-183. https://dx.doi.org/10.1016/j.jcis.2021.03.051

MLA:

Kieserling, Helena, et al. "Structure and adsorption behavior of high hydrostatic pressure-treated β-lactoglobulin." Journal of Colloid and Interface Science 596 (2021): 173-183.

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