Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry
Berlamont H, De Witte C, De Bruyckere S, Fox JG, Backert S, Smet A, Boyen F, Haesebrouck F (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 10
Journal Issue: 3
DOI: 10.3390/pathogens10030366
Abstract
Gastric helicobacters (Helicobacter (H.) pylori and non-H. pylori Helicobacter species (NHPHs)) colonize the stomach of humans and/or animals. Helicobacter species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, Helicobacter species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric Helicobacter isolates of 10 different Helicobacter species using MALDI-TOF MS in order to establish a more elaborate Helicobacter reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores >= 2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related Helicobacter species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric Helicobacter species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account.
Authors with CRIS profile
Involved external institutions
Universiteit Gent (UGent) / Ghent University
Belgium (BE)
University of Antwerp / Universiteit Antwerpen
Belgium (BE)
Massachusetts Institute of Technology (MIT)
United States (USA) (US)
Belgium (BE)
University of Antwerp / Universiteit Antwerpen
Belgium (BE)
Massachusetts Institute of Technology (MIT)
United States (USA) (US)
How to cite
APA:
Berlamont, H., De Witte, C., De Bruyckere, S., Fox, J.G., Backert, S., Smet, A.,... Haesebrouck, F. (2021). Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry. Pathogens, 10(3). https://dx.doi.org/10.3390/pathogens10030366
MLA:
Berlamont, Helena, et al. "Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry." Pathogens 10.3 (2021).
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