Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum

Möller J, Nosratabadi F, Musella L, Hofmann J, Burkovski A (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 9

Article Number: 14

Journal Issue: 1

DOI: 10.3390/proteomes9010014

Abstract

Host-pathogen interactions are often studied in vitro using primary or immortal cell lines. This set-up avoids ethical problems of animal testing and has the additional advantage of lower costs. However, the influence of cell culture media on bacterial growth and metabolism is not considered or investigated in most cases. To address this question growth and proteome adaptation of Corynebacterium diphtheriae strain ISS3319 were investigated in this study. Bacteria were cultured in standard growth medium, cell culture medium, and fetal calf serum. Mass spectrometric analyses and label-free protein quantification hint at an increased bacterial pathogenicity when grown in cell culture medium as well as an influence of the growth medium on the cell envelope.

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How to cite

APA:

Möller, J., Nosratabadi, F., Musella, L., Hofmann, J., & Burkovski, A. (2021). Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum. Proteomes, 9(1). https://dx.doi.org/10.3390/proteomes9010014

MLA:

Möller, Jens, et al. "Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum." Proteomes 9.1 (2021).

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