Neuronal differentiation from induced pluripotent stem cell-derived neurospheres by the application of oxidized alginate-gelatin-laminin hydrogels

Distler T, Lauria I, Detsch R, Sauter CM, Bendt F, Kapr J, Ruetten S, Boccaccini AR, Fritsche E (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Biomedicines MDPI AG

Book Volume: 9

Article Number: 261

Journal Issue: 3

DOI: 10.3390/biomedicines9030261

Abstract

Biodegradable hydrogels that promote stem cell differentiation into neurons in three di-mensions (3D) are highly desired in biomedical research to study drug neurotoxicity or to yield cell-containing biomaterials for neuronal tissue repair. Here, we demonstrate that oxidized alginate-gelatin-laminin (ADA-GEL-LAM) hydrogels facilitate neuronal differentiation and growth of em-bedded human induced pluripotent stem cell (hiPSC) derived neurospheres. ADA-GEL and ADA- GEL-LAM hydrogels exhibiting a stiffness close to ~5 kPa at initial cell culture conditions of 37 °C were prepared. Laminin supplemented ADA-GEL promoted an increase in neuronal differentiation in comparison to pristine ADA-GEL, with enhanced neuron migration from the neurospheres to the bulk 3D hydrogel matrix. The presence of laminin in ADA-GEL led to a more than two-fold increase in the number of neurospheres with migrated neurons. Our findings suggest that laminin addition to oxidized alginate—gelatin hydrogel matrices plays a crucial role to tailor oxidized alginate-gela-tin hydrogels suitable for 3D neuronal cell culture applications.

Authors with CRIS profile

Thomas Distler Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Leibniz-Institut für umweltmedizinische Forschung (IUF) / Leibniz Research Institute for Environmental Medicine DE Germany (DE) Universitätsklinikum Aachen (UKA) DE Germany (DE)

How to cite

APA:

Distler, T., Lauria, I., Detsch, R., Sauter, C.M., Bendt, F., Kapr, J.,... Fritsche, E. (2021). Neuronal differentiation from induced pluripotent stem cell-derived neurospheres by the application of oxidized alginate-gelatin-laminin hydrogels. Biomedicines, 9(3). https://doi.org/10.3390/biomedicines9030261

MLA:

Distler, Thomas, et al. "Neuronal differentiation from induced pluripotent stem cell-derived neurospheres by the application of oxidized alginate-gelatin-laminin hydrogels." Biomedicines 9.3 (2021).

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