From Submerged Cultures to 3D Cell Culture Models: Evolution of Nasal Epithelial Cells in Asthma Research and Virus Infection
Aydin M, Naumova EA, Bellm A, Behrendt AK, Giachero F, Bahlmann N, Zhang W, Wirth S, Paulsen F, Arnold WH, Ehrhardt A (2021)
Publication Type: Journal article, Review article
Publication year: 2021
Journal
Book Volume: 13
Journal Issue: 3
DOI: 10.3390/v13030387
Abstract
Understanding the response to viral infection in the context of respiratory diseases is of significant importance. Recently, there has been more focus on the role of the nasal epithelium in disease modeling. Here, we provide an overview of different submerged, organotypic 3D and spheroid cell culture models of nasal epithelial cells, which were used to study asthma and allergy with a special focus on virus infection. In detail, this review summarizes the importance, benefits, and disadvantages of patient-derived cell culture models of nasal- and bronchial epithelial cells, including a comparison of these cell culture models and a discussion on why investigators should consider using nasal epithelial cells in their research. Exposure experiments, simple virus transduction analyses as well as genetic studies can be performed in these models, which may provide first insights into the complexity of molecular signatures and may open new doors for drug discovery and biomarker research.
Authors with CRIS profile
Involved external institutions
Universität Witten/Herdecke
Germany (DE)
Universitätsklinikum Aachen (UKA)
Germany (DE)
Universitätsmedizin Greifswald / Universitätsklinikum Greifswald
Germany (DE)
HELIOS Kliniken
Germany (DE)
Germany (DE)
Universitätsklinikum Aachen (UKA)
Germany (DE)
Universitätsmedizin Greifswald / Universitätsklinikum Greifswald
Germany (DE)
HELIOS Kliniken
Germany (DE)
How to cite
APA:
Aydin, M., Naumova, E.A., Bellm, A., Behrendt, A.K., Giachero, F., Bahlmann, N.,... Ehrhardt, A. (2021). From Submerged Cultures to 3D Cell Culture Models: Evolution of Nasal Epithelial Cells in Asthma Research and Virus Infection. Viruses, 13(3). https://dx.doi.org/10.3390/v13030387
MLA:
Aydin, Malik, et al. "From Submerged Cultures to 3D Cell Culture Models: Evolution of Nasal Epithelial Cells in Asthma Research and Virus Infection." Viruses 13.3 (2021).
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