Cellular spion uptake and toxicity in various head and neck cancer cell lines

Balk M, Haus T, Band J, Unterweger H, Schreiber E, Friedrich RP, Alexiou C, Gostian AO (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Nanomaterials MDPI

Book Volume: 11

Pages Range: 1-19

Article Number: 726

Journal Issue: 3

DOI: 10.3390/nano11030726

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) feature distinct magnetic properties that make them useful and effective tools for various diagnostic, therapeutic and theranostic applica-tions. In particular, their use in magnetic drug targeting (MDT) promises to be an effective approach for the treatment of various diseases such as cancer. At the cellular level, SPION uptake, along with SPION-mediated toxicity, represents the most important prerequisite for successful application. Thus, the present study determines SPION uptake, toxicity and biocompatibility in human head and neck tumor cell lines of the tongue, pharynx and salivary gland. Using magnetic susceptibility measurements, microscopy, atomic emission spectroscopy, flow cytometry, and plasma coagulation, we analyzed the magnetic properties, cellular uptake and biocompatibility of two different SPION types in the presence and absence of external magnetic fields. Incubation of cells with lauric acid and human serum albumin-coated nanoparticles (SPION^LA-HSA) resulted in substantial particle uptake with low cytotoxicity. In contrast, uptake of lauric acid-coated nanoparticles (SPION^LA) was sub-stantially increased but accompanied by higher toxicity. The presence of an external magnetic field significantly increased cellular uptake of both particles, although cytotoxicity was not significantly increased in any of the cell lines. SPIONs coated with lauric acid and/or human serum albumin show different patterns of uptake and toxicity in response to an external magnetic field. Consequently, the results indicate the potential use of SPIONs as vehicles for MDT in head and neck cancer.

Authors with CRIS profile

Harald Unterweger Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christoph Alexiou Professur für Nanomedizin

How to cite

APA:

Balk, M., Haus, T., Band, J., Unterweger, H., Schreiber, E., Friedrich, R.P.,... Gostian, A.O. (2021). Cellular spion uptake and toxicity in various head and neck cancer cell lines. Nanomaterials, 11(3), 1-19. https://doi.org/10.3390/nano11030726

MLA:

Balk, Matthias, et al. "Cellular spion uptake and toxicity in various head and neck cancer cell lines." Nanomaterials 11.3 (2021): 1-19.

BibTeX: Download