The Coxiella burnetii effector protein CaeB modulates endoplasmatic reticulum (ER) stress signalling and is required for efficient replication in Galleria mellonella

Friedrich A, Beare PA, Schulze-Luehrmann J, Cordsmeier A, Pazen T, Sonnewald S, Lührmann A (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Cellular Microbiology Wiley

DOI: 10.1111/cmi.13305

Abstract

The obligate intracellular pathogen Coxiella burnetii is the causative agent of the zoonosis Q fever. C. burnetii infection can have severe outcomes due to the development of chronic infection. To establish and maintain an infection, C. burnetii depends on a functional type IVB secretion system (T4BSS) and, thus, on the translocation of effector proteins into the host cell. Here, we showed that the C. burnetii T4BSS effector protein CaeB targets the conserved endoplasmatic reticulum (ER) stress sensor IRE1 during ER stress in mammalian and plant cells. CaeB-induced upregulation of IRE1 RNase activity was essential for CaeB-mediated inhibition of ER stress-induced cell death. Our data reveal a novel role for CaeB in ER stress signalling modulation and demonstrate that CaeB is involved in pathogenicity in vivo. Furthermore, we provide evidence that C. burnetii infection leads to modulation of the ER stress sensors IRE1 and PERK, but not ATF6 during ER stress. While the upregulation of the RNase activity of IRE1 during ER stress depends on CaeB, modulation of PERK is CaeB independent, suggesting that C. burnetii encodes several factors influencing ER stress during infection.

Authors with CRIS profile

Anja Friedrich Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Arne Cordsmeier Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Tobias Pazen Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Sophia Sonnewald Lehrstuhl für Biochemie Anja Lührmann Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene

Involved external institutions

US National Institutes of Health (NIH) US United States (USA) (US)

How to cite

APA:

Friedrich, A., Beare, P.A., Schulze-Luehrmann, J., Cordsmeier, A., Pazen, T., Sonnewald, S., & Lührmann, A. (2021). The Coxiella burnetii effector protein CaeB modulates endoplasmatic reticulum (ER) stress signalling and is required for efficient replication in Galleria mellonella. Cellular Microbiology. https://dx.doi.org/10.1111/cmi.13305

MLA:

Friedrich, Anja, et al. "The Coxiella burnetii effector protein CaeB modulates endoplasmatic reticulum (ER) stress signalling and is required for efficient replication in Galleria mellonella." Cellular Microbiology (2021).

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