Matricellular Protein SPARCL1 Regulates Blood Vessel Integrity and Antagonizes Inflammatory Bowel Disease

Regensburger D, Tenkerian C, Pürzer V, Schmid B, Wohlfahrt T, Stolzer I, Lopez Posadas R, Günther C, Waldner M, Becker C, Sticht H, Petter K, Flierl C, Gass T, Thoenissen T, Geppert CI, Britzen-Laurent N, Meniel VS, Ramming A, Stürzl M, Naschberger E (2021)


Publication Type: Journal article

Publication year: 2021

Journal

DOI: 10.1093/ibd/izaa346

Abstract

Background
The understanding of vascular plasticity is key to defining the role of blood vessels in physiologic and pathogenic processes. In the present study, the impact of the vascular quiescence marker SPARCL1 on angiogenesis, capillary morphogenesis, and vessel integrity was evaluated.

Methods
Angiogenesis was studied using the metatarsal test, an ex vivo model of sprouting angiogenesis. In addition, acute and chronic dextran sodium sulfate colitis models with SPARCL1 knockout mice were applied.

Results
This approach indicated that SPARCL1 inhibits angiogenesis and supports vessel morphogenesis and integrity. Evidence was provided that SPARCL1-mediated stabilization of vessel integrity counteracts vessel permeability and inflammation in acute and chronic dextran sodium sulfate colitis models. Structure-function analyses of purified SPARCL1 identified the acidic domain of the protein necessary for its anti-angiogenic activity.

Conclusions
Our findings inaugurate SPARCL1 as a blood vessel–derived anti-angiogenic molecule required for vessel morphogenesis and integrity. SPARCL1 opens new perspectives as a vascular marker of susceptibility to colitis and as a therapeutic molecule to support blood vessel stability in this disease.

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How to cite

APA:

Regensburger, D., Tenkerian, C., Pürzer, V., Schmid, B., Wohlfahrt, T., Stolzer, I.,... Naschberger, E. (2021). Matricellular Protein SPARCL1 Regulates Blood Vessel Integrity and Antagonizes Inflammatory Bowel Disease. Inflammatory Bowel Diseases. https://dx.doi.org/10.1093/ibd/izaa346

MLA:

Regensburger, Daniela, et al. "Matricellular Protein SPARCL1 Regulates Blood Vessel Integrity and Antagonizes Inflammatory Bowel Disease." Inflammatory Bowel Diseases (2021).

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