Light-Controlled Cell-Cycle Arrest and Apoptosis
Uhl E, Wolff F, Mangal S, Dube H, Zanin E (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 60
Pages Range: 1187-1196
Journal Issue: 3
Abstract
Cell-cycle interference by small molecules has widely been used to study fundamental biological mechanisms and to treat a great variety of diseases, most notably cancer. However, at present only limited possibilities exist for spatio-temporal control of the cell cycle. Here we report on a photocaging strategy to reversibly arrest the cell cycle at metaphase or induce apoptosis using blue-light irradiation. The versatile proteasome inhibitor MG132 is photocaged directly at the reactive aldehyde function effectively masking its biological activity. Upon irradiation reversible cell-cycle arrest in the metaphase is demonstrated to take place in vivo. Similarly, apoptosis can efficiently be induced by irradiation of human cancer cells. With the developed photopharmacological approach spatio-temporal control of the cell cycle is thus enabled with very high modulation, as caged MG132 shows no effect on proliferation in the dark. In addition, full compatibility of photo-controlled uncaging with dynamic microscopy techniques in vivo is demonstrated. This visible-light responsive tool should be of great value for biological as well as medicinal approaches in need of high-precision targeting of the proteasome and thereby the cell cycle and apoptosis.
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Germany (DE)How to cite
APA:
Uhl, E., Wolff, F., Mangal, S., Dube, H., & Zanin, E. (2021). Light-Controlled Cell-Cycle Arrest and Apoptosis. Angewandte Chemie International Edition, 60(3), 1187-1196. https://dx.doi.org/10.1002/anie.202008267
MLA:
Uhl, Edgar, et al. "Light-Controlled Cell-Cycle Arrest and Apoptosis." Angewandte Chemie International Edition 60.3 (2021): 1187-1196.
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