Lipid Dynamics in Membranes Slowed Down by Transmembrane Proteins
Ebersberger L, Schindler T, Kirsch S, Pluhackova K, Schambony A, Seydel T, Böckmann R, Unruh T (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 8
DOI: 10.3389/fcell.2020.579388
Abstract
Lipids and proteins, as essential components of biological cell membranes, exhibit a significant degree of freedom for different kinds of motions including lateral long-range mobility. Due to their interactions, they not only preserve the cellular membrane but also contribute to many important cellular functions as e.g., signal transport or molecular exchange of the cell with its surrounding. Many of these processes take place on a short time (up to some nanoseconds) and length scale (up to some nanometers) which is perfectly accessible by quasielastic neutron scattering (QENS) experiments and molecular dynamics (MD) simulations. In order to probe the influence of a peptide, a transmembrane sequence of the transferrin receptor (TFRC) protein, on the dynamics of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) large unilamellar vesicles (LUVs) on a nanosecond time scale, high-resolution QENS experiments and complementary MD simulations have been utilized. By using different scattering contrasts in the experiment (chain-deuterated lipids and protonated lipids, respectively), a model could be developed which allows to examine the lipid and peptide dynamics separately. The experimental results revealed a restricted lipid lateral mobility in the presence of the TFRC transmembrane peptides. Also the apparent self-diffusion coefficient of the lateral movement of the peptide molecules could be determined quantitatively for the probed short-time regime. The findings could be confirmed very precisely by MD simulations. Furthermore, the article presents an estimation for the radius of influence of the peptides on the lipid long-range dynamics which could be determined by consistently combining results from experiment and simulation.
Authors with CRIS profile
Lisa Ebersberger
Professur für Nanomaterialcharakterisierung (Streumethoden)
Torben Schindler
Lehrstuhl für Kristallographie und Strukturphysik
Sonja Kirsch
Professur für Computational Biology
Kristyna Pluhackova
Professur für Computational Biology
Alexandra Schambony
Lehrstuhl für Entwicklungsbiologie
Rainer Böckmann
Professur für Computational Biology
Tobias Unruh
Professur für Nanomaterialcharakterisierung (Streumethoden)
Additional Organisation(s)
Involved external institutions
France (FR)How to cite
APA:
Ebersberger, L., Schindler, T., Kirsch, S., Pluhackova, K., Schambony, A., Seydel, T.,... Unruh, T. (2020). Lipid Dynamics in Membranes Slowed Down by Transmembrane Proteins. Frontiers in Cell and Developmental Biology, 8. https://dx.doi.org/10.3389/fcell.2020.579388
MLA:
Ebersberger, Lisa, et al. "Lipid Dynamics in Membranes Slowed Down by Transmembrane Proteins." Frontiers in Cell and Developmental Biology 8 (2020).
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