High-Precision Protein-Tracking With Interferometric Scattering Microscopy

Taylor RW, Holler C, Mahmoodabadi RG, Küppers M, Mirzaalian Dastjerdi H, Zaburdaev V, Schambony A, Sandoghdar V (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Book Volume: 8

Article Number: 590158

DOI: 10.3389/fcell.2020.590158

Abstract

The mobility of proteins and lipids within the cell, sculpted oftentimes by the organization of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behavior. Interferometric scattering (iSCAT) microscopy is an emerging technique well-suited for visualizing the diffusion of gold nanoparticle-labeled membrane proteins to a spatial and temporal resolution beyond the means of traditional fluorescent labels. We discuss the applicability of interferometric single particle tracking (iSPT) microscopy to investigate the minutia in the motion of a protein through measurements visualizing the mobility of the epidermal growth factor receptor in various biological scenarios on the live cell.

Authors with CRIS profile

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light Germany (DE)

How to cite

APA:

Taylor, R.W., Holler, C., Mahmoodabadi, R.G., Küppers, M., Mirzaalian Dastjerdi, H., Zaburdaev, V.,... Sandoghdar, V. (2020). High-Precision Protein-Tracking With Interferometric Scattering Microscopy. Frontiers in Cell and Developmental Biology, 8. https://dx.doi.org/10.3389/fcell.2020.590158

MLA:

Taylor, Richard W., et al. "High-Precision Protein-Tracking With Interferometric Scattering Microscopy." Frontiers in Cell and Developmental Biology 8 (2020).

BibTeX: Download