14-3-3ε protein-loaded 3D hydrogels favor osteogenesis

Aldana AA, Uhart M, Abraham GA, Bustos DM, Boccaccini AR (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Journal of Materials Science: Materials in Medicine Springer

Book Volume: 31

Article Number: 105

Journal Issue: 11

DOI: 10.1007/s10856-020-06434-1

Abstract

3D printing has emerged as vanguard technique of biofabrication to assemble cells, biomaterials and biomolecules in a spatially controlled manner to reproduce native tissues. In this work, gelatin methacrylate (GelMA)/alginate hydrogel scaffolds were obtained by 3D printing and 14-3-3ε protein was encapsulated in the hydrogel to induce osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASC). GelMA/alginate-based grid-like structures were printed and remained stable upon photo-crosslinking. The viscosity of alginate allowed to control the pore size and strand width. A higher viscosity of hydrogel ink enhanced the printing accuracy. Protein-loaded GelMA/alginate-based hydrogel showed a clear induction of the osteogenic differentiation of hASC cells. The results are relevant for future developments of GelMA/alginate for bone tissue engineering given the positive effect of 14-3-3ε protein on both cell adhesion and proliferation.

Authors with CRIS profile

Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Laboratorio de Integración de Señales Celulares AR Argentina (AR) Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA) AR Argentina (AR)

How to cite

APA:

Aldana, A.A., Uhart, M., Abraham, G.A., Bustos, D.M., & Boccaccini, A.R. (2020). 14-3-3ε protein-loaded 3D hydrogels favor osteogenesis. Journal of Materials Science: Materials in Medicine, 31(11). https://dx.doi.org/10.1007/s10856-020-06434-1

MLA:

Aldana, Ana A., et al. "14-3-3ε protein-loaded 3D hydrogels favor osteogenesis." Journal of Materials Science: Materials in Medicine 31.11 (2020).

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