Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development

Langheinrich M, Wirtz S, Kneis B, Gittler MM, Tyc O, Schierwagen R, Brunner M, Krautz C, Pilarsky C, Trebicka J, Agaimy A, Grützmann R, Kersting S, Weber G (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Journal of Clinical Medicine MDPI

Book Volume: 9

Journal Issue: 9

DOI: 10.3390/jcm9092785

Abstract

Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of Enterococcus in patients undergoing preoperative stent placement in the common bile duct, (iii) significant differences in the beta diversity between patients who developed a postoperative pancreatic fistula (POPF B/C), (iv) patients with POPF B/C were more likely to have bacteria belonging to the genus Enterococcus, and (v) differences in microbiome composition with regard to the pancreatic gland texture. The structure of the microbiome is distinctive in different compartments, and can be associated with the development of a postoperative pancreatic fistula.

Authors with CRIS profile

Stefan Wirtz Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Maximilian Brunner Lehrstuhl für Allgemein- und Viszeralchirurgie Christian Krautz Department of Surgery Christian Pilarsky Professur für Chirurgische Forschung Abbas Agaimy Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Robert Grützmann Department of Surgery Georg Weber Department of Surgery

Involved external institutions

Universitätsklinikum Frankfurt am Main (KGU) DE Germany (DE)

How to cite

APA:

Langheinrich, M., Wirtz, S., Kneis, B., Gittler, M.M., Tyc, O., Schierwagen, R.,... Weber, G. (2020). Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development. Journal of Clinical Medicine, 9(9). https://dx.doi.org/10.3390/jcm9092785

MLA:

Langheinrich, Melanie, et al. "Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development." Journal of Clinical Medicine 9.9 (2020).

BibTeX: Download