Terbium Excitation Spectroscopy as a Detection Method for Chromatographic Separation of Lanthanide-Binding Biomolecules
Jurkowski W, Heilmann M, Becker A, Buchholz R, Brueck T (2020)
Publication Language: English
Publication Type: Journal article, Original article
Publication year: 2020
Journal
Book Volume: 5
Pages Range: 27050-27056
Journal Issue: 42
Abstract
Studies of biosorption and bioaccumulation of
heavy metals deal mostly with challenging, inhomogeneous, and
complex materials. Therefore, most reports describe only
application studies, while fundamental research is limited to
indirect methods and speculations on the binding mechanisms. In
this study, we describe a method for detecting and isolating heavy
metal-binding biomolecules directly from crude extracts. The
underlying principle is terbium sensitization and fluorescence
excitation spectroscopy used offline after a chromatographic run.
Compounds interacting with metal ions inevitably change the
coordination sphere of terbium, which is reflected in the excitation
spectrum leading to metal-specific luminescence. Main advantages
of our approach include simple, fast, and inexpensive experiment
design, nondestructive measurements, and detection limits far below 1 mg. Here, we have applied our method for three promising
biosorbents (green algae, moss, and cyanobacterium) and obtained first information on the character of active compounds isolated
from each species.
Authors with CRIS profile
Involved external institutions
Germany (DE)How to cite
APA:
Jurkowski, W., Heilmann, M., Becker, A., Buchholz, R., & Brueck, T. (2020). Terbium Excitation Spectroscopy as a Detection Method for Chromatographic Separation of Lanthanide-Binding Biomolecules. ACS Omega, 5(42), 27050-27056. https://dx.doi.org/10.1021/acsomega.0c02135
MLA:
Jurkowski, Wojciech, et al. "Terbium Excitation Spectroscopy as a Detection Method for Chromatographic Separation of Lanthanide-Binding Biomolecules." ACS Omega 5.42 (2020): 27050-27056.
BibTeX: Download