Späth M, Hohmann M, Roider C, Lengenfelder B, Stelzle F, Wirtz S, Klämpfl F (2020)
Publication Type: Journal article
Publication year: 2020
Book Volume: 10
Article Number: 17391
Issue: 1
DOI: 10.1038/s41598-020-74447-5
Due to significant advantages, the trend in the field of medical technology is moving towards minimally or even non-invasive examination methods. In this respect, optical methods offer inherent benefits, as does diffuse reflectance imaging (DRI). The present study attempts to prove the suitability of DRI—when implemented alongside a suitable setup and data evaluation algorithm—to derive information from anatomically correctly scaled human capillaries (diameter: 10μm, length: 45μm) by conducting extensive Monte–Carlo simulations and by verifying the findings through laboratory experiments. As a result, the method of shifted position-diffuse reflectance imaging (SP-DRI) is established by which average signal modulations of up to 5% could be generated with an illumination wavelength of λ=424nm and a core diameter of the illumination fiber of 50μm. No reference image is needed for this technique. The present study reveals that the diffuse reflectance data in combination with the SP-DRI normalization are suitable to localize human capillaries within turbid media.
APA:
Späth, M., Hohmann, M., Roider, C., Lengenfelder, B., Stelzle, F., Wirtz, S., & Klämpfl, F. (2020). Towards shifted position-diffuse reflectance imaging of anatomically correctly scaled human microvasculature. Scientific Reports, 10. https://dx.doi.org/10.1038/s41598-020-74447-5
MLA:
Späth, Moritz, et al. "Towards shifted position-diffuse reflectance imaging of anatomically correctly scaled human microvasculature." Scientific Reports 10 (2020).
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