Synthesis and characterization of citrate-stabilized gold-coated superparamagnetic iron oxide nanoparticles for biomedical applications

Stein R, Friedrich B, Mühlberger M, Cebulla N, Schreiber E, Tietze R, Cicha I, Alexiou C, Dutz S, Boccaccini AR, Unterweger H (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Molecules MDPI

Book Volume: 25

Article Number: 4425

Journal Issue: 19

DOI: 10.3390/molecules25194425

Abstract

Surface-functionalized gold-coated superparamagnetic iron oxide nanoparticles (Au-SPIONs) may be a useful tool in various biomedical applications. To obtain Au-SPIONs, gold salt was precipitated onto citrate-stabilized SPIONs (Cit-SPIONs) using a simple, aqueous one-pot technique inspired by the Turkevich method of gold nanoparticle synthesis. By the further stabilization of the Au-SPION surface with additional citrate (Cit-Au-SPIONs), controllable and reproducible Z-averages enhanced long-term dispersion stability and moderate dispersion pH values were achieved. The citrate concentration of the reaction solution and the gold/iron ratio was found to have a major influence on the particle characteristics. While the gold-coating reduced the saturation magnetization to 40.7% in comparison to pure Cit-SPIONs, the superparamagnetic behavior of Cit-Au-SPIONs was maintained. The formation of nanosized gold on the SPION surface was confirmed by X-ray diffraction measurements. Cit-Au-SPION concentrations of up to 100 µg Fe/mL for 48 h had no cytotoxic effect on Jurkat cells. At a particle concentration of 100 µg Fe/mL, Jurkat cells were found to take up Cit-Au-SPIONs after 24 h of incubation. A significantly higher attachment of thiol-containing L-cysteine to the particle surface was observed for Cit-Au-SPIONs (53%) in comparison to pure Cit-SPIONs (7%).

Authors with CRIS profile

René Stein Department of Otorhinolaryngology – Head and Neck Surgery Bernhard Friedrich Department of Otorhinolaryngology – Head and Neck Surgery Marina Mühlberger Department of Otorhinolaryngology – Head and Neck Surgery Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery Iwona Cicha Medizinische Fakultät Christoph Alexiou Professur für Nanomedizin Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Harald Unterweger Department of Otorhinolaryngology – Head and Neck Surgery

Involved external institutions

Technische Universität Ilmenau DE Germany (DE)

How to cite

APA:

Stein, R., Friedrich, B., Mühlberger, M., Cebulla, N., Schreiber, E., Tietze, R.,... Unterweger, H. (2020). Synthesis and characterization of citrate-stabilized gold-coated superparamagnetic iron oxide nanoparticles for biomedical applications. Molecules, 25(19). https://dx.doi.org/10.3390/molecules25194425

MLA:

Stein, René, et al. "Synthesis and characterization of citrate-stabilized gold-coated superparamagnetic iron oxide nanoparticles for biomedical applications." Molecules 25.19 (2020).

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