Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
Tenzer S, Docter D, Kuharev J, Musyanovych A, Fetz V, Hecht R, Schlenk F, Fischer D, Kiouptsi K, Reinhardt C, Landfester K, Schild H, Maskos M, Knauer SK, Stauber RH (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 8
Pages Range: 772-781
Journal Issue: 10
Abstract
In biological fluids, proteins bind to the surface of nanoparticles to form a coating known as the protein corona, which can critically affect the interaction of the nanoparticles with living systems. As physiological systems are highly dynamic, it is important to obtain a time-resolved knowledge of protein-corona formation, development and biological relevancy. Here we show that label-free snapshot proteomics can be used to obtain quantitative time-resolved profiles of human plasma coronas formed on silica and polystyrene nanoparticles of various size and surface functionalization. Complex time- and nanoparticle-specific coronas, which comprise almost 300 different proteins, were found to form rapidly (<0.5 minutes) and, over time, to change significantly in terms of the amount of bound protein, but not in composition. Rapid corona formation is found to affect haemolysis, thrombocyte activation, nanoparticle uptake and endothelial cell death at an early exposure time. © 2013 Macmillan Publishers Limited. All rights reserved.
Authors with CRIS profile
Involved external institutions
Universitätsmedizin der Johannes Gutenberg-Universität Mainz
Germany (DE)
Max-Planck-Institut für Polymerforschung (MPI-P) / Max Planck Institute for Polymer Research
Germany (DE)
Mainz Institute of Microtechnology / Institut für Mikrotechnik Mainz (IMM)
Germany (DE)
Universität Duisburg-Essen (UDE)
Germany (DE)
Friedrich-Schiller-Universität Jena
Germany (DE)
Germany (DE)
Max-Planck-Institut für Polymerforschung (MPI-P) / Max Planck Institute for Polymer Research
Germany (DE)
Mainz Institute of Microtechnology / Institut für Mikrotechnik Mainz (IMM)
Germany (DE)
Universität Duisburg-Essen (UDE)
Germany (DE)
Friedrich-Schiller-Universität Jena
Germany (DE)
How to cite
APA:
Tenzer, S., Docter, D., Kuharev, J., Musyanovych, A., Fetz, V., Hecht, R.,... Stauber, R.H. (2013). Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. Nature Nanotechnology, 8(10), 772-781. https://dx.doi.org/10.1038/nnano.2013.181
MLA:
Tenzer, Stefan, et al. "Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology." Nature Nanotechnology 8.10 (2013): 772-781.
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