Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(Methacrylamide)s
Cokca C, Zartner L, Tabujew I, Fischer D, Peneva K (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 41
Article Number: 1900668
Journal Issue: 6
Abstract
A highly efficient transfection agent is reported that is based on terpolymer consisting of N-(2-hydroxypropyl)methacrylamide (HPMA), N-(3-guanidinopropyl) methacrylamide (GPMA), and N-(2-indolethyl)methacrylamide monomers (IEMA) by analogy to the amphipathic cell-penetrating peptides containing tryptophan and arginine residues. The incorporation of the indole-bearing monomer leads to successful plasmid DNA condensation even at a nitrogen-to-phosphate (N/P) ratio of 1. The hydrodynamic diameter of polyplexes is determined to be below 200 nm for all N/P ratios. The transfection studies demonstrate a 200-fold increase of the transgene expression in comparison to P(HPMA-co-GPMA) with the same guanidinium content. This study reveals the strong potential of the indole group as a side-chain pendant group that can increase the cellular uptake of polymers and the transfection efficiency of the respective polyplexes.
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Germany (DE)How to cite
APA:
Cokca, C., Zartner, L., Tabujew, I., Fischer, D., & Peneva, K. (2020). Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(Methacrylamide)s. Macromolecular Rapid Communications, 41(6). https://dx.doi.org/10.1002/marc.201900668
MLA:
Cokca, Ceren, et al. "Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(Methacrylamide)s." Macromolecular Rapid Communications 41.6 (2020).
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