HLA-G and HLA-F protein isoform expression in breast cancer patients receiving neoadjuvant treatment

Würfel F, Hübner H, Häberle L, Gaß P, Hein A, Jud S, Hack C, Wunderle M, Schulz-Wendtland R, Erber R, Hartmann A, Ekici AB, Beckmann M, Fasching P, Rübner M (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 10

Article Number: 15750

Journal Issue: 1

DOI: 10.1038/s41598-020-72837-3

Abstract

The immunosuppressive human leukocyte antigens HLA-G and HLA-F are expressed on trophoblast and malignant cells. Four membrane-bound and three soluble HLA-G protein isoforms have been described, which have different immunosuppressive potentials. HLA-F has three transcript variants, resulting in three different protein isoforms. The aim of this study was to evaluate the prognostic and predictive value of HLA-G and HLA-F protein isoform expression patterns in patients with breast cancer. Core biopsies were taken at diagnosis in patients with HER2+ (n = 28), luminal B-like (n = 49) and triple-negative (n = 38) breast cancers who received neoadjuvant chemotherapy. Expression levels of HLA-F and -G were correlated with the pathological complete response (pCR). Protein expression was determined by Western blot analysis, using two antibodies for each HLA, specific for different isoforms. The protein expression of HLA isoforms did not significantly differ between breast cancer subtypes. However, some initial indications were found for an association between the soluble HLA-G6 protein isoform and pCR in HER2+ breast cancer. The study provides preliminary evidence for the evaluation of HLA-G isoform expression, in particular HLA-G6, as a possible new marker for pCR in HER2+ breast cancer.

Authors with CRIS profile

Franziska Würfel Professur für Translationale Frauenheilkunde und Geburtshilfe Hanna Hübner Professur für Experimentelle Tumorpathologie Paul Gaß Department of Obstetrics and Gynaecology Alexander Hein Department of Obstetrics and Gynaecology Sebastian Jud Department of Obstetrics and Gynaecology Carolin Hack Medizinische Fakultät Marius Wunderle Department of Obstetrics and Gynaecology Ramona Erber Institute of Pathology Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Arif Bülent Ekici Institute of Human Genetics Matthias Beckmann Lehrstuhl für Geburtshilfe und Frauenheilkunde Peter Fasching Professur für Translationale Frauenheilkunde und Geburtshilfe Matthias Rübner Department of Obstetrics and Gynaecology

How to cite

APA:

Würfel, F., Hübner, H., Häberle, L., Gaß, P., Hein, A., Jud, S.,... Rübner, M. (2020). HLA-G and HLA-F protein isoform expression in breast cancer patients receiving neoadjuvant treatment. Scientific Reports, 10(1). https://dx.doi.org/10.1038/s41598-020-72837-3

MLA:

Würfel, Franziska, et al. "HLA-G and HLA-F protein isoform expression in breast cancer patients receiving neoadjuvant treatment." Scientific Reports 10.1 (2020).

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