Preconditioning of bioactive glasses before introduction to static cell culture: What is really necessary?

Hohenbild F, Arango Ospina M, Moghaddam A, Boccaccini AR, Westhauser F (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Methods and Protocols MDPI

Book Volume: 3

Pages Range: 1-12

Article Number: 38

Journal Issue: 2

DOI: 10.3390/mps3020038

Abstract

Due to their high bioreactivity, the in-vitro analysis of bioactive glasses (BGs) can be challenging when it comes to maintaining a physiological pH. To improve BG biocompatibility, a heterogenic spectrum of preconditioning approaches, such as “passivation” of the BGs by incubation in cell culture medium, are used but have never been directly compared. In this study, the effect of passivation periods of up to 72 h on pH alkalization and viability of human bone marrow-derived mesenchymal stromal cells was evaluated to determine a time-efficient passivation protocol using granules based on the 45S5-BG composition (in wt%: 45.0 SiO2, 24.5 Na2 O, 24.5 CaO, 6.0 P2 O5) in different concentrations. pH alkalization was most reduced after passivation of 24 h. Cell viability continuously improved with increasing passivation time being significantly higher after passivation of at least 24 h compared to non-passivated 45S5-BG and the necessary passivation time increased with increasing BG concentrations. In this setting, a passivation period of 24 h presented as an effective approach to provide a biocompatible cell culture setting. In conclusion, before introduction of BGs in cell culture, different passivation periods should be evaluated in order to meet the respective experimental settings, e.g., by following the experimental protocols used in this study.

Authors with CRIS profile

Marcela Arango Ospina Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Universitätsklinikum Heidelberg DE Germany (DE) Klinikum Aschaffenburg DE Germany (DE)

How to cite

APA:

Hohenbild, F., Arango Ospina, M., Moghaddam, A., Boccaccini, A.R., & Westhauser, F. (2020). Preconditioning of bioactive glasses before introduction to static cell culture: What is really necessary? Methods and Protocols, 3(2), 1-12. https://dx.doi.org/10.3390/mps3020038

MLA:

Hohenbild, Frederike, et al. "Preconditioning of bioactive glasses before introduction to static cell culture: What is really necessary?" Methods and Protocols 3.2 (2020): 1-12.

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