Auxetic nuclei in embryonic stem cells exiting pluripotency
Pagliara S, Franze K, Mcclain CR, Wylde G, Fisher CL, Franklin RJM, Kabla AJ, Keyser UF, Chalut KJ (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 13
Pages Range: 638-644
Journal Issue: 6
DOI: 10.1038/nmat3943
Abstract
Embryonic stem cells (ESCs) self-renew in a state of naïve pluripotency in which they are competent to generate all somatic cells. It has been hypothesized that, before irreversibly committing, ESCs pass through at least one metastable transition state. This transition would represent a gateway for differentiation and reprogramming of somatic cells. Here, we show that during the transition, the nuclei of ESCs are auxetic: they exhibit a cross-sectional expansion when stretched and a cross-sectional contraction when compressed, and their stiffness increases under compression. We also show that the auxetic phenotype of transition ESC nuclei is driven at least in part by global chromatin decondensation. Through the regulation of molecular turnover in the differentiating nucleus by external forces, auxeticity could be a key element in mechanotransduction. Our findings highlight the importance of nuclear structure in the regulation of differentiation and reprogramming. © 2014 Macmillan Publishers Limited.
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APA:
Pagliara, S., Franze, K., Mcclain, C.R., Wylde, G., Fisher, C.L., Franklin, R.J.M.,... Chalut, K.J. (2014). Auxetic nuclei in embryonic stem cells exiting pluripotency. Nature Materials, 13(6), 638-644. https://dx.doi.org/10.1038/nmat3943
MLA:
Pagliara, Stefano, et al. "Auxetic nuclei in embryonic stem cells exiting pluripotency." Nature Materials 13.6 (2014): 638-644.
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