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Secretome analysis of mesenchymal stem cell factors fostering oligodendroglial differentiation of neural stem cells in vivo

Agrelo IS, Schira-Heinen J, Beyer F, Groh J, Bütermann C, Estrada V, Poschmann G, Bribian A, Jadasz JJ, Lopez-Mascaraque L, Kremer D, Martini R, Müller HW, Hartung HP, Adjaye J, Stühler K, Küry P (2020)

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Publication Type: Journal article

Publication year: 2020

Journal

Book Volume: 21

Pages Range: 1-25

Article Number: 4350

Journal Issue: 12

DOI: 10.3390/ijms21124350

Abstract

Mesenchymal stem cell (MSC)-secreted factors have been shown to significantly promote oligodendrogenesis from cultured primary adult neural stem cells (aNSCs) and oligodendroglial precursor cells (OPCs). Revealing underlying mechanisms of how aNSCs can be fostered to differentiate into a specific cell lineage could provide important insights for the establishment of novel neuroregenerative treatment approaches aiming at myelin repair. However, the nature of MSC-derived differentiation and maturation factors acting on the oligodendroglial lineage has not been identified thus far. In addition to missing information on active ingredients, the degree to which MSC-dependent lineage instruction is functional in vivo also remains to be established. We here demonstrate that MSC-derived factors can indeed stimulate oligodendrogenesis and myelin sheath generation of aNSCs transplanted into different rodent central nervous system (CNS) regions, and furthermore, we provide insights into the underlying mechanism on the basis of a comparative mass spectrometry secretome analysis. We identified a number of secreted proteins known to act on oligodendroglia lineage differentiation. Among them, the tissue inhibitor of metalloproteinase type 1 (TIMP-1) was revealed to be an active component of the MSC-conditioned medium, thus validating our chosen secretome approach.

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APA:

Agrelo, I.S., Schira-Heinen, J., Beyer, F., Groh, J., Bütermann, C., Estrada, V.,... Küry, P. (2020). Secretome analysis of mesenchymal stem cell factors fostering oligodendroglial differentiation of neural stem cells in vivo. International Journal of Molecular Sciences, 21(12), 1-25. https://dx.doi.org/10.3390/ijms21124350

MLA:

Agrelo, Iria Samper, et al. "Secretome analysis of mesenchymal stem cell factors fostering oligodendroglial differentiation of neural stem cells in vivo." International Journal of Molecular Sciences 21.12 (2020): 1-25.

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