FOXA1 Gene Expression for Defining Molecular Subtypes of Muscle-Invasive Bladder Cancer after Radical Cystectomy

Sikic D, Eckstein M, Wirtz RM, Jarczyk J, Worst TS, Porubsky S, Keck B, Kunath F, Weyerer V, Breyer J, Otto W, Rinaldetti S, Bolenz C, Hartmann A, Wullich B, Erben P (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Journal of Clinical Medicine MDPI

Book Volume: 9

Journal Issue: 4

DOI: 10.3390/jcm9040994

Abstract

It remains unclear how to implement the recently revealed basal and luminal subtypes of muscle-invasive bladder cancer (MIBC) into daily clinical routine and whether molecular marker panels can be reduced. The mRNA expression of basal (KRT5) and luminal (FOXA1, GATA3, KRT20) markers was measured by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and correlated to clinicopathological features, recurrence-free survival (RFS), disease-free survival (DFS), and overall survival (OS) in 80 patients with MIBC who underwent radical cystectomy. Additionally, the correlation of single markers with the basal and non-basal subtypes defined by a 36-gene panel was examined and then validated in the TCGA (The Cancer Genome Atlas) cohort. High expression of FOXA1 (p = 0.0048) and KRT20 (p = 0.0317) was associated with reduced RFS. In the multivariable analysis, only FOXA1 remained an independent prognostic marker for DFS (p = 0.0333) and RFS (p = 0.0310). FOXA1 expression (AUC = 0.79; p = 0.0007) was closest to the combined marker expression (AUC = 0.79; p = 0.0015) in resembling the non-basal subtype defined by the 36-gene panel. FOXA1 in combination with KRT5 may be used to distinguish the basal and non-basal subtypes of MIBC.

Authors with CRIS profile

Markus Eckstein Institute of Pathology Bastian Keck Medizinische Fakultät Frank Kunath Urologische Klinik Veronika Bahlinger Institute of Pathology Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Bernd Wullich Lehrstuhl für Urologie

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Universitätsklinikum Ulm DE Germany (DE) STRATIFYER Molecular Pathology GmbH DE Germany (DE) Universität Regensburg DE Germany (DE)

How to cite

APA:

Sikic, D., Eckstein, M., Wirtz, R.M., Jarczyk, J., Worst, T.S., Porubsky, S.,... Erben, P. (2020). FOXA1 Gene Expression for Defining Molecular Subtypes of Muscle-Invasive Bladder Cancer after Radical Cystectomy. Journal of Clinical Medicine, 9(4). https://doi.org/10.3390/jcm9040994

MLA:

Sikic, Danijel, et al. "FOXA1 Gene Expression for Defining Molecular Subtypes of Muscle-Invasive Bladder Cancer after Radical Cystectomy." Journal of Clinical Medicine 9.4 (2020).

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