The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist
Domsch K, Schröder J, Janeschik M, Schaub C, Lohmann I (2021)
Publication Status: In review
Publication Type: Journal article, Report
Future Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 35
Pages Range: 108577
Journal Issue: 1
URI: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)31566-7
DOI: 10.1016/j.celrep.2020.108577
Open Access Link: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)31566-7
Abstract
Early lineage-specific master regulators are essential for the specification of cell types. However, once cells are committed to a specific fate, it is critical to restrict the activity of such factors to enable differentiation. To date, it remains unclear how these factors are silenced. Using the Drosophila mesoderm as a model and a comparative genomic approach, we identify the Hox transcription factor Ultrabithorax (Ubx) to be critical for the repression of the master regulator Twist. Mesoderm-specific Ubx loss-of-function experiments using CRISPR-Cas9 and overexpression studies demonstrate that Ubx majorly impacts twist transcription. A mechanistic analysis reveals that Ubx requires the NK-homeodomain protein Tinman to bind to the twist promoter. Furthermore, we find these factor interactions to be critical for silencing by recruiting the Polycomb DNA binding protein Pleiohomeotic. Altogether, our data reveal that Ubx is a critical player in mediating the silencing of Twist, which is crucial for coordinated muscle differentiation.
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Germany (DE)How to cite
APA:
Domsch, K., Schröder, J., Janeschik, M., Schaub, C., & Lohmann, I. (2021). The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist. Cell Reports, 35(1), 108577. https://dx.doi.org/10.1016/j.celrep.2020.108577
MLA:
Domsch, Katrin, et al. "The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist." Cell Reports 35.1 (2021): 108577.
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