Chimeric FLS2 receptors reveal the basis for differential flagellin perception in arabidopsis and tomato

Mueller K, Bittel P, Chinchilla D, Jehle AK, Albert M, Boller T, Felix G (2012)

Publication Type: Journal article

Publication year: 2012

Journal

The Plant Cell American Society of Plant Biologists

Book Volume: 24

Pages Range: 2213-2224

Journal Issue: 5

DOI: 10.1105/tpc.112.096073

Abstract

The flagellin receptor of Arabidopsis thaliana, At-FLAGELLIN SENSING2 (FLS2), has become a model for mechanistic and functional studies on plant immune receptors. Here, we started out with a comparison of At-FLS2 and the orthologous tomato (Solanum lycopersicum) receptor Sl-FLS2. Both receptors specifically responded to picomolar concentrations of the genuine flg22 ligand but proved insensitive to >106-fold higher concentrations of CLV3 peptides that have recently been reported as a second type of ligand for At-FLS2. At-FLS2 and Sl-FLS2 exhibit species-specific differences in the recognition of shortened or sequence-modified flg22 ligands. To map the sites responsible for these species-specific traits on the FLS2 receptors, we performed domain swaps, substituting subsets of the 28 leucine-rich repeats (LRRs) in At-FLS2 with the corresponding LRRs from Sl-FLS2. We found that the LRRs 7 to 10 of Sl-FLS2 determine the high affinity of Sl-FLS2 for the core part RINSAKDD of flg22. In addition, we discovered importance of the LRRs 19 to 24 for the responsiveness to C-terminally modified flagellin peptides. These results indicate that ligand perception in FLS2 is a complex molecular process that involves LRRs from both the outermost and innermost LRRs of the FLS2 ectodomain. © 2012 American Society of Plant Biologists. All rights reserved.

Authors with CRIS profile

Markus Albert

Additional Organisation(s)

Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

Universität Basel CH Switzerland (CH) Eberhard Karls Universität Tübingen DE Germany (DE)

How to cite

APA:

Mueller, K., Bittel, P., Chinchilla, D., Jehle, A.K., Albert, M., Boller, T., & Felix, G. (2012). Chimeric FLS2 receptors reveal the basis for differential flagellin perception in arabidopsis and tomato. The Plant Cell, 24(5), 2213-2224. https://dx.doi.org/10.1105/tpc.112.096073

MLA:

Mueller, Katharina, et al. "Chimeric FLS2 receptors reveal the basis for differential flagellin perception in arabidopsis and tomato." The Plant Cell 24.5 (2012): 2213-2224.

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