A two-hybrid-receptor assay demonstrates heteromer formation as switch-on for plant immune receptors

Albert M, Jehle AK, Fuerst U, Chinchilla D, Boller T, Felix G (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Plant Physiology American Society of Plant Biologists

Book Volume: 163

Pages Range: 1504-1509

Journal Issue: 4

DOI: 10.1104/pp.113.227736

Abstract

Receptor kinases sense extracellular signals and trigger intracellular signaling and physiological responses. However, how does signal binding to the extracellular domain activate the cytoplasmic kinase domain? Activation of the plant immunoreceptor Flagellin sensing2 (FLS2) by its bacterial ligand flagellin or the peptide-epitope flg22 coincides with rapid complex formation with a second receptor kinase termed brassinosteroid receptor1 associated kinase1 (BAK1). Here, we show that the receptor pair of FLS2 and BAK1 is also functional when the roles of the complex partners are reversed by swapping their cytosolic domains. This reciprocal constellation prevents interference by redundant partners that can partially substitute for BAK1 and demonstrates that formation of the heteromeric complex is the molecular switch for transmembrane signaling. A similar approach with swaps between the Elongation factor-Tu receptor and BAK1 also resulted in a functional receptor/coreceptor pair, suggesting that a "two-hybrid-receptor assay" is of more general use for studying heteromeric receptor complexes. © 2013 American Society of Plant Biologists. All Rights Reserved.

Authors with CRIS profile

Markus Albert

Additional Organisation(s)

Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

Eberhard Karls Universität Tübingen DE Germany (DE) Universität Basel CH Switzerland (CH)

How to cite

APA:

Albert, M., Jehle, A.K., Fuerst, U., Chinchilla, D., Boller, T., & Felix, G. (2013). A two-hybrid-receptor assay demonstrates heteromer formation as switch-on for plant immune receptors. Plant Physiology, 163(4), 1504-1509. https://dx.doi.org/10.1104/pp.113.227736

MLA:

Albert, Markus, et al. "A two-hybrid-receptor assay demonstrates heteromer formation as switch-on for plant immune receptors." Plant Physiology 163.4 (2013): 1504-1509.

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