A Facile One-Pot Synthesis of Water-Soluble, Patchy Fe3O4-Au Nanoparticles for Application in Radiation Therapy

Klein S, Hubner J, Menter C, Distel L, Neuhuber W, Kryschi C (2019)


Publication Type: Journal article

Publication year: 2019

Journal

Book Volume: 9

Journal Issue: 1

DOI: 10.3390/app9010015

Abstract

A facile one-pot synthesis route for the preparation of water-soluble, biocompatible patchy Fe3O4-Au nanoparticles (Fe3O4-Au pNPs) was developed. Biocompatibility was attained through surface functionalization with 1-methyl-3-(dodecylphosphonic acid) imidazolium bromide. The morphology, composition, crystal structure and magnetic properties of the Fe3O4-Au pNPs were investigated by conducting experiments with transmission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and superconducting quantum interference device, respectively. Internalization of the Fe3O4-Au pNPs by MCF-7 cells occurred via endocytosis. The performance of the Fe3O4-Au pNPs as X-ray radiosensitizer in tumor cells was compared with that of gold nanocluster and Fe3O4 NPs. For this reason, MCF-7, A549 and MCF-10A cells were loaded with the respective kind of nanoparticles and treated with X-rays at doses of 1, 2 or 3 Gy. The nanoparticle-induced changes of the concentration of the reactive oxygen species (ROS) were detected using specific assays, and the cell survival under X-ray exposure was assessed employing the clonogenic assay. In comparison with the gold nanocluster and Fe3O4 NPs, the Fe3O4-Au pNPs exhibited the highest catalytic capacity for ROS generation in MCF-7 and A549 cells, whereas in the X-ray-induced ROS formation in healthy MCF-10A cells was hardly enhanced by the Fe3O4 NPs and Fe3O4-Au pNPs. Moreover, the excellent performance of Fe3O4-Au pNPs as X-ray radiosensitizers was verified by the quickly decaying radiation dose survival curve of the nanoparticle-loaded MCF-7 and A549 cells and corroborated by the small values of the associated dose-modifying factors.

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APA:

Klein, S., Hubner, J., Menter, C., Distel, L., Neuhuber, W., & Kryschi, C. (2019). A Facile One-Pot Synthesis of Water-Soluble, Patchy Fe3O4-Au Nanoparticles for Application in Radiation Therapy. Applied Sciences, 9(1). https://dx.doi.org/10.3390/app9010015

MLA:

Klein, Stefanie, et al. "A Facile One-Pot Synthesis of Water-Soluble, Patchy Fe3O4-Au Nanoparticles for Application in Radiation Therapy." Applied Sciences 9.1 (2019).

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