Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces

Weisenstein C, Schaar D, Wigger AK, Schäfer-Eberwein H, Boßerhoff AK, Bolívar PH (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Biomedical Optics Express Optical Society of America

Book Volume: 11

Pages Range: 448-460

Journal Issue: 1

DOI: 10.1364/BOE.380818

Abstract

THz technologies are a powerful tool for label-free detection of biomolecules. However, significant reduction of the lower detection limit is required to apply THz-sensors in biomedical diagnosis. This paper reports an ultrasensitive THz-biosensor based on asymmetric double split ring resonators (aDSRR) for the direct label- and PCR-free detection of DNA at physiologically relevant concentrations. We introduce selective functionalization and localized electric field concentration to enhance aDSRR sensitivity and specificity. The sensor characteristics are demonstrated using the human tumor marker MIA in cDNA samples produced from total RNA without PCR-amplification. Measurements of DNA samples with concentrations as low as 1.55 × 10−12 mol/l are presented.

Authors with CRIS profile

Dominik Schaar Lehrstuhl für Biochemie und Molekulare Medizin Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Involved external institutions

Universität Siegen DE Germany (DE)

How to cite

APA:

Weisenstein, C., Schaar, D., Wigger, A.K., Schäfer-Eberwein, H., Boßerhoff, A.K., & Bolívar, P.H. (2020). Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces. Biomedical Optics Express, 11(1), 448-460. https://dx.doi.org/10.1364/BOE.380818

MLA:

Weisenstein, Christian, et al. "Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces." Biomedical Optics Express 11.1 (2020): 448-460.

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