Isolation of β-carotene, α-carotene and lutein from carrots by countercurrent chromatography with the solvent system modifier benzotrifluoride
Englert M, Hammann S, Vetter W (2015)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2015
Journal
Publisher: Elsevier
Book Volume: 1388
Pages Range: 119-125
URI: https://www.sciencedirect.com/science/article/pii/S0021967315002459
DOI: 10.1016/j.chroma.2015.02.020
Abstract
A carotenoid purification method with dual-mode countercurrent chromatography (CCC) for β-carotene, α-carotene and lutein from a fresh carrot extract was developed. The fluorinated liquid benzotrifluoride (IUPAC name: (trifluoromethyl)benzene) was used as a novel modifier in the non-aqueous ternary solvent system n-hexane/benzotrifluoride/acetonitrile. The ternary phase diagram of the type I solvent system was used to select two-phase solvent mixtures which enabled an efficient preparative separation of α-carotene, β-carotene and lutein from concomitant pigments in crude carrot extract. By means of the modifier, high separation factors (α ≥ 1.2) were obtained, allowing baseline resolution between α-carotene and β-carotene due to specific chemical interactions such as π–π molecular interactions. After optimizing the injection step with a pseudo-ternary phase diagram, 51 mg of β-carotene, 32 mg of α-carotene and 4 mg of lutein could be isolated from 100.2 mg crude carrot extract in a short time and with high purities of 95% and 99% by using dual-mode CCC, respectively. Temperatures >22 °C had a negative impact on the separation of α-carotene and β-carotene.
Authors with CRIS profile
Simon Hammann
Juniorprofessur für Lebensmittelsicherheit und -qualität (am Henriette Schmidt-Burkhardt Lehrstuhl)
Involved external institutions
Germany (DE)How to cite
APA:
Englert, M., Hammann, S., & Vetter, W. (2015). Isolation of β-carotene, α-carotene and lutein from carrots by countercurrent chromatography with the solvent system modifier benzotrifluoride. Journal of Chromatography A, 1388, 119-125. https://dx.doi.org/10.1016/j.chroma.2015.02.020
MLA:
Englert, Michael, Simon Hammann, and Walter Vetter. "Isolation of β-carotene, α-carotene and lutein from carrots by countercurrent chromatography with the solvent system modifier benzotrifluoride." Journal of Chromatography A 1388 (2015): 119-125.
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