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Structure and dynamics of interfacial peptides and proteins from vibrational sum-frequency generation spectroscopy

Hosseinpour S, Roeters S, Bonn M, Peukert W, Woutersen S, Weidner T (2020)

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Publication Language: English

Publication Type: Journal article, Review article

Publication year: 2020

Journal

Book Volume: 120

Pages Range: 3420-3465

Journal Issue: 7

URI: https://pubs.acs.org/doi/abs/10.1021/acs.chemrev.9b00410

DOI: 10.1021/acs.chemrev.9b00410

Abstract

Proteins at interfaces play an important role in cell biology, immunology, bioengineering, and biomimetic material design. Many biological processes are based on interfacial protein action, ranging from cellular communication to immune responses and the protein-driven mineralization of bone. Despite the importance of interfacial proteins, comparatively little is known about their structure. The standard methods for studying crystalline or solution-phase proteins (X-ray diffraction and NMR) are not well suited for studying proteins at interfaces, and for these proteins, we still lack a corresponding technique that can provide the same level of structural resolutions. This is not surprising in view of the challenges involved in probing the structure of proteins within monomolecular films assembled at a very thin interface in situ. Vibrational sum-frequency generation (SFG) spectroscopy has the potential to overcome this ‘resolution gap’ and investigate the structure and dynamics of proteins at interfaces at the molecular level and with sub-picosecond time resolution. While SFG studies were initially limited to simple model peptides, the past decade has seen a dramatic advancement of experimental techniques and data analysis methods, which has made it possible to also study interfacial proteins and their folding, binding, orientation, hydration, and dynamics. In this review, we first explain the principles of protein SFG and the experimental and theoretical methods to measure and analyze protein SFG spectra. Then we give an extensive overview of the interfacial proteins studied thus far with SFG. We highlight representative examples to demonstrate recent advances in probing the structure of proteins at the interfaces of liquids, membranes, minerals, and synthetic materials.

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How to cite

APA:

Hosseinpour, S., Roeters, S., Bonn, M., Peukert, W., Woutersen, S., & Weidner, T. (2020). Structure and dynamics of interfacial peptides and proteins from vibrational sum-frequency generation spectroscopy. Chemical Reviews, 120(7), 3420-3465. https://dx.doi.org/10.1021/acs.chemrev.9b00410

MLA:

Hosseinpour, Saman, et al. "Structure and dynamics of interfacial peptides and proteins from vibrational sum-frequency generation spectroscopy." Chemical Reviews 120.7 (2020): 3420-3465.

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