Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis
Sato Y, Hilbert L, Oda H, Wan Y, Heddleston JM, Chew TL, Zaburdaev V, Keller P, Lionnet T, Vastenhouw N, Kimura H (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 146
Journal Issue: 19
DOI: 10.1242/dev.179127
Abstract
Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with alpha-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms.
Authors with CRIS profile
Involved external institutions
Tokyo Institute of Technology
Japan (JP)
Howard Hughes Medical Institute
United States (USA) (US)
Center for Systems Biology Dresden (CSBD)
Germany (DE)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
NYU Langone Medical Center
United States (USA) (US)
Japan (JP)
Howard Hughes Medical Institute
United States (USA) (US)
Center for Systems Biology Dresden (CSBD)
Germany (DE)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
NYU Langone Medical Center
United States (USA) (US)
How to cite
APA:
Sato, Y., Hilbert, L., Oda, H., Wan, Y., Heddleston, J.M., Chew, T.-L.,... Kimura, H. (2019). Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis. Development, 146(19). https://doi.org/10.1242/dev.179127
MLA:
Sato, Yuko, et al. "Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis." Development 146.19 (2019).
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