Suitability of ultrasound-guided fine-needle aspiration biopsy for transcriptome sequencing of the canine prostate

Thiemeyer H, Taher L, Schille JT, Harder L, Hungerbuehler SO, Mischke R, Hewicker-Trautwein M, Kiełbowicz Z, Brenig B, Schütz E, Beck J, Murua Escobar H, Nolte I (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 9

Pages Range: 13216-

Journal Issue: 1

DOI: 10.1038/s41598-019-49271-1

Abstract

Ultrasound-guided fine-needle aspiration (US-FNA) biopsy is a widely used minimally invasive sampling procedure for cytological diagnosis. This study investigates the feasibility of using US-FNA samples for both cytological diagnosis and whole transcriptome RNA-sequencing analysis (RNA-Seq), with the ultimate aim of improving canine prostate cancer management. The feasibility of the US-FNA procedure was evaluated intra vitam on 43 dogs. Additionally, aspirates from 31 euthanised dogs were collected for standardising the procedure. Each aspirate was separated into two subsamples: for cytology and RNA extraction. Additional prostate tissue samples served as control for RNA quantity and quality evaluation, and differential expression analysis. The US-FNA sampling procedure was feasible in 95% of dogs. RNA isolation of US-FNA samples was successfully performed using phenol-chloroform extraction. The extracted RNA of 56% of a subset of US-FNA samples met the quality requirements for RNA-Seq. Expression analysis revealed that only 153 genes were exclusively differentially expressed between non-malignant US-FNAs and tissues. Moreover, only 36 differentially expressed genes were associated with the US-FNA sampling technique and unrelated to the diagnosis. Furthermore, the gene expression profiles clearly distinguished between non-malignant and malignant samples. This proves US-FNA to be useful for molecular profiling.

Authors with CRIS profile

Leila Taher Juniorprofessur für Bioinformatik

Involved external institutions

Stiftung Tierärztliche Hochschule Hannover (TiHo) DE Germany (DE) Chronix Biomedical GmbH DE Germany (DE) Georg-August-Universität Göttingen DE Germany (DE) Wrocław Medical University / Uniwersytet Medyczny we Wrocławiu PL Poland (PL)

How to cite

APA:

Thiemeyer, H., Taher, L., Schille, J.T., Harder, L., Hungerbuehler, S.O., Mischke, R.,... Nolte, I. (2019). Suitability of ultrasound-guided fine-needle aspiration biopsy for transcriptome sequencing of the canine prostate. Scientific Reports, 9(1), 13216-. https://doi.org/10.1038/s41598-019-49271-1

MLA:

Thiemeyer, H., et al. "Suitability of ultrasound-guided fine-needle aspiration biopsy for transcriptome sequencing of the canine prostate." Scientific Reports 9.1 (2019): 13216-.

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