Influence of the polydispersity of pH 2 and pH 3.5 beta-lactoglobulin amyloid fibril solutions on analytical methods

Heyn TR, Garamus VM, Neumann HR, Uttinger M, Guckeisen T, Heuer M, Selhuber-Unkel C, Peukert W, Keppler JK (2019)

Publication Type: Journal article

Publication year: 2019

Journal

European Polymer Journal Elsevier

Book Volume: 120

Article Number: 109211

DOI: 10.1016/j.eurpolymj.2019.08.038

Abstract

It is well known that amyloid beta-lactoglobulin (BLG) fibril solutions contain a heterogeneous mixture of amyloid aggregates and non-amyloid material. However, few information are available on how strongly separated fractions of different morphologies (straight fibrils at pH 2 and worm-like aggregates at pH 3.5) vary with respect to physicochemical properties and building blocks as most analyses are conducted with unfractionized solutions where superposition effects occur. The pH-value shift resulted in an altered degree of acid hydrolysis which led to dissimilar building blocks of the aggregates (peptides at pH 2, non-hydrolyzed protein at pH 3.5). The respective separated amyloid and non-amyloid fractions showed significantly different size (SAXS, SEC, AUC) and charge properties (Zeta potential) than the whole samples. Strong superposition effects were evident with common analyses such as FTIR, TRP fluorescence and Thioflavin-T. At the same time, structural differences of pH 2 and pH 3.5 aggregates could be presented more clearly.

Authors with CRIS profile

Maximilian Uttinger Lehrstuhl für Feststoff- und Grenzflächenverfahrenstechnik Tobias Guckeisen Lehrstuhl für Feststoff- und Grenzflächenverfahrenstechnik Wolfgang Peukert Lehrstuhl für Feststoff- und Grenzflächenverfahrenstechnik

Involved external institutions

Christian-Albrechts-Universität zu Kiel DE Germany (DE) Helmholtz-Zentrum Geesthacht Zentrum für Material- und Küstenforschung GmbH (HZG) DE Germany (DE) Wageningen University & Research NL Netherlands (NL)

How to cite

APA:

Heyn, T.R., Garamus, V.M., Neumann, H.R., Uttinger, M., Guckeisen, T., Heuer, M.,... Keppler, J.K. (2019). Influence of the polydispersity of pH 2 and pH 3.5 beta-lactoglobulin amyloid fibril solutions on analytical methods. European Polymer Journal, 120. https://dx.doi.org/10.1016/j.eurpolymj.2019.08.038

MLA:

Heyn, Timon R., et al. "Influence of the polydispersity of pH 2 and pH 3.5 beta-lactoglobulin amyloid fibril solutions on analytical methods." European Polymer Journal 120 (2019).

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