Establishment of serine protease htrA mutants in Helicobacter pylori is associated with secA mutations

Zawilak-Pawlik A, Zarzecka U, Żyła-Uklejewicz D, Lach J, Strapagiel D, Tegtmeyer-Backert N, Böhm M, Backert S, Skorko-Glonek J (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 9

Article Number: 11794

Journal Issue: 1

DOI: 10.1038/s41598-019-48030-6

Abstract

Helicobacter pylori plays an essential role in the pathogenesis of gastritis, peptic ulcer disease, and gastric cancer. The serine protease HtrA, an important secreted virulence factor, disrupts the gastric epithelium, which enables H. pylori to transmigrate across the epithelium and inject the oncogenic CagA protein into host cells. The function of periplasmic HtrA for the H. pylori cell is unknown, mainly due to unavailability of the htrA mutants. In fact, htrA has been described as an essential gene in this bacterium. We have screened 100 worldwide H. pylori isolates and show that only in the N6 strain it was possible to delete htrA or mutate the htrA gene to produce proteolytically inactive HtrA. We have sequenced the wild-type and mutant chromosomes and we found that inactivation of htrA is associated with mutations in SecA – a component of the Sec translocon apparatus used to translocate proteins from the cytoplasm into the periplasm. The cooperation of SecA and HtrA has been already suggested in Streptococcus pneumonia, in which these two proteins co-localize. Hence, our results pinpointing a potential functional relationship between HtrA and the Sec translocon in H. pylori possibly indicate for the more general mechanism responsible to maintain bacterial periplasmic homeostasis.

Authors with CRIS profile

Nicole Tegtmeyer-Backert Lehrstuhl für Mikrobiologie Manja Böhm Lehrstuhl für Mikrobiologie Steffen Backert Lehrstuhl für Mikrobiologie

Involved external institutions

Konsorcjum BBMRI.pl PL Poland (PL) University of Gdańsk / Uniwersytet Gdański PL Poland (PL) Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences PL Poland (PL)

How to cite

APA:

Zawilak-Pawlik, A., Zarzecka, U., Żyła-Uklejewicz, D., Lach, J., Strapagiel, D., Tegtmeyer-Backert, N.,... Skorko-Glonek, J. (2019). Establishment of serine protease htrA mutants in Helicobacter pylori is associated with secA mutations. Scientific Reports, 9(1). https://dx.doi.org/10.1038/s41598-019-48030-6

MLA:

Zawilak-Pawlik, Anna, et al. "Establishment of serine protease htrA mutants in Helicobacter pylori is associated with secA mutations." Scientific Reports 9.1 (2019).

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