Röhrig W, Achenbach S, Deutsch B, Pischetsrieder M (2019)
Publication Status: Published
Publication Type: Journal article
Publication year: 2019
Book Volume: 60
Pages Range: 1475-1488
Journal Issue: 8
URI: http://www.jlr.org/content/60/8/1475.full
DOI: 10.1194/jlr.D094680
Open Access Link: http://www.jlr.org/content/60/8/1475.full
Endocannabinoids and endocannabinoid-related compounds (ERCs) are involved in many physiological processes.
They are released on demand from phosphoinositide and N-acylphosphatidyl ethanolamine (NAPE) precursors and comprise 2-monoacylglycerols (2-MGs) and FA ethanolamides (FEAs). Despite the abundance of advanced quantitative methods, however, their determined concentrations in blood plasma are inconsistent because 2-MGs and FEAs undergo artifactual de novo formation, chemical isomerization, and degradation during sample collection and storage. For a comprehensive survey of these compounds in blood and plasma, we have developed and validated an ultra-HPLC-MS/MS method to quantify 24 endocannabinoids, ERCs, and their phospholipid precursors. Immediate acidification of EDTA-blood to pH 5.8 blocked artifactual FEA formation for at least 4 h on ice. The 2-MGs were stabilized after plasma harvest with 0.5 M potassium thiocyanate at pH 4.7. FEA and MG plasma concentrations in six healthy volunteers ranged between 0.04-3.48 and 0.63-6.18 ng/ml, respectively. Interestingly, only 1-5% of circulating FEAs were present in their free form, while the majority was bound to NAPEs. Similarly, 97% of 2-arachidonoylglycerol (2-AG) was bound to a potential phosphoinositide pool. The herein-described stabilization and extraction methods may now be used to reliably and comprehensively quantify endocannabinoids, ERCs, and their phospholipid precursors in clinical studies.
APA:
Röhrig, W., Achenbach, S., Deutsch, B., & Pischetsrieder, M. (2019). Quantification of 24 circulating endocannabinoids, endocannabinoid-related compounds, and their phospholipid precursors in human plasma by UHPLC-MS/MS. Journal of Lipid Research, 60(8), 1475-1488. https://dx.doi.org/10.1194/jlr.D094680
MLA:
Röhrig, Waldemar, et al. "Quantification of 24 circulating endocannabinoids, endocannabinoid-related compounds, and their phospholipid precursors in human plasma by UHPLC-MS/MS." Journal of Lipid Research 60.8 (2019): 1475-1488.
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