Dalabasmaz S, Dittrich D, Kellner I, Drewello T, Pischetsrieder M (2019)
Publication Status: Published
Publication Type: Journal article
Publication year: 2019
Book Volume: 207
Pages Range: 103444
URI: https://authors.elsevier.com/a/1ZVHp6gB-WXHON
DOI: 10.1016/j.jprot.2019.103444
Proteolysis during the storage of UHT milk is associated with major technological problems, particularly bitter
off-flavors and age gelation limiting the shelf life of milk. In this study, untargeted peptide profiling by MALDITOF-
MS identified peptides that were formed by proteolysis and reflected the storage of UHT milk. Analysis of
nine different commercial UHT samples recorded peptide profiles during and at the end of their shelf life.
Relative quantification and sequencing of the peptides revealed that the concentrations of 22 peptides increased
significantly during the storage of UHT milk due to the activity of endogenous milk proteases and microbial
proteases as well as other unidentified proteolytic mechanisms. Based on highly discriminative AUC values from
receiver operator characteristic (ROC) curve analysis, we selected ten peptides as marker candidates. Among
those, the peptide β-casein192–206 (m/z 1668.9) was the most suitable marker differentiating expired-UHT from
regular-UHT samples with 100% accuracy. Additionally, β-casein191–206 (m/z 1782.0) showed 100% specificity
and β-casein139–161 (m/z 2696.4) 100% sensitivity. Thus, β-casein192–206, either by itself or in combination with
β-casein191–206 and β-casein139–161, presents a reliable marker to monitor the storage of UHT milk based on
proteolytic mechanisms.
APA:
Dalabasmaz, S., Dittrich, D., Kellner, I., Drewello, T., & Pischetsrieder, M. (2019). Identification of peptides reflecting the storage of UHT milk by MALDI-TOF-MS peptide profiling. Journal of Proteomics, 207, 103444. https://dx.doi.org/10.1016/j.jprot.2019.103444
MLA:
Dalabasmaz, Sevim, et al. "Identification of peptides reflecting the storage of UHT milk by MALDI-TOF-MS peptide profiling." Journal of Proteomics 207 (2019): 103444.
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