Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata

Brault ML, Petit JD, Immel F, Nicolas WJ, Glavier M, Brocard L, Gaston A, Fouché M, Hawkins TJ, Crowet JM, Grison MS, Germain V, Rocher M, Kraner M, Alva V, Claverol S, Paterlini A, Helariutta Y, Deleu M, Lins L, Tilsner J, Bayer EM (2019)

Publication Type: Journal article

Publication year: 2019

Journal

EMBO Reports EMBO Press

Article Number: e47182

DOI: 10.15252/embr.201847182

Abstract

In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)–plasma membrane (PM) contacts coincide with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and transmembrane region protein (MCTP) family, key regulators of cell-to-cell signalling in plants, act as ER-PM tethers specifically at plasmodesmata. We report that MCTPs are plasmodesmata proteins that insert into the ER via their transmembrane region while their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss of function mutant induces plant developmental defects, impaired plasmodesmata function and composition, while MCTP4 expression in a yeast Δtether mutant partially restores ER-PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER-PM contacts and cell-to-cell signalling.

Authors with CRIS profile

Max Kraner Lehrstuhl für Biochemie

Involved external institutions

Université de Bordeaux FR France (FR) Durham University GB United Kingdom (GB) University of Liège (ULg) / Université de Liège BE Belgium (BE) Max-Planck-Institut für Entwicklungsbiologie / Max Planck Institute for Developmental Biology DE Germany (DE) University of Cambridge GB United Kingdom (GB) University of St Andrews GB United Kingdom (GB)

How to cite

APA:

Brault, M.L., Petit, J.D., Immel, F., Nicolas, W.J., Glavier, M., Brocard, L.,... Bayer, E.M. (2019). Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Reports. https://dx.doi.org/10.15252/embr.201847182

MLA:

Brault, Marie L., et al. "Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata." EMBO Reports (2019).

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