More than a toxin: Protein inventory of Clostridium tetani toxoid vaccines

Journal article


Publication Details

Author(s): Möller J, Kraner M, Burkovski A
Journal: Proteomes
Publication year: 2019
Volume: 7
Journal issue: 2
ISSN: 2227-7382


Abstract

Clostridium tetani is the etiological agent of tetanus, a life-threatening bacterial infection. The most efficient protection strategy against tetanus is a vaccination with the C. tetani neurotoxin, which is inactivated by formaldehyde-crosslinking. Since we assumed that besides the tetanus toxin, other proteins of C. tetani may also be present in toxoid preparations, we analyzed commercially available vaccines from different countries in respect to their protein content using mass spectrometry. In total 991 proteins could be identified in all five analyzed vaccines, 206 proteins were common in all analyzed vaccines and 54 proteins from the 206 proteins were potential antigens. The additionally present proteins may contribute at least partially to protection against C. tetani infection by supporting the function of the vaccine against the devastating effects of the tetanus toxin indirectly. Two different label-free protein quantification methods were applied for an estimation of protein contents. Similar results were obtained with a Total Protein Approach (TPA)-based method and Protein Discoverer 2.2 software package based on the minora algorithm. Depending on the tetanus toxoid vaccine and the quantification method used, tetanus neurotoxin contributes between 14 and 76 % to the total C. tetani protein content and varying numbers of other C. tetani proteins were detected.


FAU Authors / FAU Editors

Burkovski, Andreas Prof. Dr.
Professur für Mikrobiologie
Sonderforschungsbereich 796 (mit integriertem Graduiertenkolleg) Steuerungsmechanismen mikrobieller Effektoren in Wirtszellen
Kraner, Max Dr.
Möller, Jens
Lehrstuhl für Mikrobiologie


How to cite

APA:
Möller, J., Kraner, M., & Burkovski, A. (2019). More than a toxin: Protein inventory of Clostridium tetani toxoid vaccines. Proteomes, 7(2). https://dx.doi.org/10.3390/proteomes7020015

MLA:
Möller, Jens, Max Kraner, and Andreas Burkovski. "More than a toxin: Protein inventory of Clostridium tetani toxoid vaccines." Proteomes 7.2 (2019).

BibTeX: 

Last updated on 2019-18-06 at 19:08