Properties of the HtrA Protease From Bacterium Helicobacter pylori Whose Activity Is Indispensable for Growth Under Stress Conditions

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Zarzecka U, Modrak-Wojcik A, Figaj D, Apanowicz M, Lesner A, Bzowska A, Lipinska B, Zawilak-Pawlik A, Backert S, Skorko-Glonek J
Zeitschrift: Frontiers in Microbiology
Jahr der Veröffentlichung: 2019
Band: 10
ISSN: 1664-302X


Abstract

The protease high temperature requirement A from the gastric pathogen Helicobacter pylori (HtrA(Hp)) belongs to the well conserved family of serine proteases. HtrA(Hp) is an important secreted virulence factor involved in the disruption of tight and adherens junctions during infection. Very little is known about the function of HtrA(Hp) in the H. pylori cell physiology due to the lack of htrA knockout strains. Here, using a newly constructed Delta htrA mutant strain, we found that bacteria deprived of HtrA(Hp) showed increased sensitivity to certain types of stress, including elevated temperature, pH and osmotic shock, as well as treatment with puromycin. These data indicate that HtrA(Hp) plays a protective role in the H. pylori cell, presumably associated with maintenance of important periplasmic and outer membrane proteins. Purified HtrA(Hp) was shown to be very tolerant to a wide range of temperature and pH values. Remarkably, the protein exhibited a very high thermal stability with the melting point (T-m) values of above 85 degrees C. Moreover, HtrA(Hp) showed the capability to regain its active structure following treatment under denaturing conditions. Taken together, our work demonstrates that HtrA is well adapted to operate under harsh conditions as an exported virulence factor, but also inside the bacterial cell as an important component of the protein quality control system in the stressed cellular envelope.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Backert, Steffen Prof. Dr.
Lehrstuhl für Mikrobiologie


Einrichtungen weiterer Autorinnen und Autoren

Polska Akademia Nauk (PAN) / Polish Academy of Sciences
University of Gdańsk / Uniwersytet Gdański
University of Warsaw / Uniwersytet Warszawski


Zitierweisen

APA:
Zarzecka, U., Modrak-Wojcik, A., Figaj, D., Apanowicz, M., Lesner, A., Bzowska, A.,... Skorko-Glonek, J. (2019). Properties of the HtrA Protease From Bacterium Helicobacter pylori Whose Activity Is Indispensable for Growth Under Stress Conditions. Frontiers in Microbiology, 10. https://dx.doi.org/10.3389/fmicb.2019.00961

MLA:
Zarzecka, Urszula, et al. "Properties of the HtrA Protease From Bacterium Helicobacter pylori Whose Activity Is Indispensable for Growth Under Stress Conditions." Frontiers in Microbiology 10 (2019).

BibTeX: 

Zuletzt aktualisiert 2019-23-05 um 08:21