Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Taylor RW, Mahmoodabadi RG, Rauschenberger V, Gießl A, Schambony A, Sandoghdar V
Zeitschrift: Nature Photonics
Jahr der Veröffentlichung: 2019
ISSN: 1749-4885


Abstract

Many of the biological functions of a cell are dictated by the intricate motion of proteins within its membrane over a spatial range of nanometres to tens of micrometres and time intervals of microseconds to minutes. This rich parameter space is not accessible by fluorescence microscopy, but it is within reach of interferometric scattering (iSCAT) particle tracking. However, as iSCAT is sensitive even to single unlabelled proteins, it is often accompanied by a large speckle-like background, which poses a substantial challenge for its application to cellular imaging. Here, we employ a new image processing approach to overcome this difficulty and demonstrate tracking of transmembrane epidermal growth factor receptors with nanometre precision in all three dimensions at up to microsecond speeds and for durations of tens of minutes. We provide examples of nanoscale motion and confinement in ubiquitous processes such as diffusion in the plasma membrane, transport on filopodia and rotational motion during endocytosis.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Gießl, Andreas PD Dr.
Lehrstuhl für Tierphysiologie
Rauschenberger, Verena
Lehrstuhl für Entwicklungsbiologie
Sandoghdar, Vahid Prof. Dr.
Lehrstuhl für Experimentalphysik (Alexander-von-Humboldt-Professur)
Schambony, Alexandra Prof. Dr.
Lehrstuhl für Entwicklungsbiologie


Einrichtungen weiterer Autorinnen und Autoren

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light


Zitierweisen

APA:
Taylor, R.W., Mahmoodabadi, R.G., Rauschenberger, V., Gießl, A., Schambony, A., & Sandoghdar, V. (2019). Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane. Nature Photonics. https://dx.doi.org/10.1038/s41566-019-0414-6

MLA:
Taylor, Richard W., et al. "Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane." Nature Photonics (2019).

BibTeX: 

Zuletzt aktualisiert 2019-10-05 um 08:38