Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds

Westhauser F, Senger AS, Obert D, Ciraldo F, Schuhladen K, Schmidmaier G, Moghaddam A, Boccaccini AR (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Journal of Tissue Engineering and Regenerative Medicine Wiley-Blackwell

Book Volume: 13

Pages Range: 179-190

Journal Issue: 2

DOI: 10.1002/term.2780

Abstract

Recent studies have demonstrated that surface characteristics, porosity, and mechanical strength of three-dimensional 45S5-type bioactive glass (BG)-based scaffolds are directly correlated with osteogenic properties. Three-dimensional BG-based scaffolds obtained from maritime natural sponges (MNSs) as sacrificial templates exhibit the required morphological properties; however, no in vivo data about the osteogenic features are available. In this study, uncoated (Group A) and gelatin-coated (Group B) crystalline MNS-obtained BG-based scaffolds were evaluated mechanically and seeded with human mesenchymal stem cells prior to subcutaneous implantation in immunodeficient mice. Before implantation and after explantation, micro-computed tomography scans were conducted, and scaffolds were finally subjected to histomorphometry. Scaffolds of both groups showed bone formation. However, Group B scaffolds performed distinctly better as indicated by a significant increase in scaffold volume (8.95%, p = 0.039) over the implantation period compared with a nonsignificant increase of 5.26% in Group A scaffolds in micro-computed tomography analysis. Furthermore, percentage bone area was 10.33% (+/- 1.18%) in the Group B scaffolds, which was significantly (p = 0.007) higher compared with the 8.53% (+/- 0.77%) in the Group A scaffolds in histomorphometry. Compressive strength was enhanced significantly by gelatin coating (9 +/- 2 vs. 4 +/- 1 MPa; p = 0.029). The presence of gelatin on the remnant parts was verified by scanning electron microscopy and X-ray spectroscopy, demonstrating the coatings' resilience. MNS-obtained BG-based scaffolds were thus confirmed to exhibit osteogenic properties in vivo that can significantly be enhanced by gelatin coating.

Authors with CRIS profile

Francesca Ciraldo Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Katharina Schuhladen Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Universitätsklinikum Heidelberg DE Germany (DE)

How to cite

APA:

Westhauser, F., Senger, A.-S., Obert, D., Ciraldo, F., Schuhladen, K., Schmidmaier, G.,... Boccaccini, A.R. (2019). Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds. Journal of Tissue Engineering and Regenerative Medicine, 13(2), 179-190. https://dx.doi.org/10.1002/term.2780

MLA:

Westhauser, Fabian, et al. "Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds." Journal of Tissue Engineering and Regenerative Medicine 13.2 (2019): 179-190.

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