Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo

Ryan EJ, Ryan AJ, Gonzalez-Vazquez A, Philippart A, Ciraldo F, Hobbs C, Nicolosi V, Boccaccini AR, Kearney CJ, O'Brien FJ (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Biomaterials Elsevier

Book Volume: 197

Pages Range: 405-416

DOI: 10.1016/j.biomaterials.2019.01.031

Abstract

The bone infection osteomyelitis (typically by Staphylococcus aureus) usually requires a multistep procedure of surgical debridement, long-term systemic high-dose antibiotics, and for larger defects bone grafting. This, combined with the alarming rise in antibiotic resistance, necessitates development of alternative approaches. Herein, we describe a one-step treatment for osteomyelitis that combines local, controlled release of non-antibiotic antibacterials with a regenerative collagen-based scaffold. To maximise efficacy, we utilised bioactive glass, an established osteoconductive material with immense capacity for bone repair, as a delivery platform for copper ions (proven antibacterial, angiogenic, and osteogenic properties). Multifunctional collagen-copper doped bioactive glass scaffolds (CuBG-CS) were fabricated with favourable microarchitectural and mechanical properties (up to 1.9-fold increase in compressive modulus over CS) within the ideal range for bone tissue engineering. Scaffolds demonstrated antibacterial activity against Staphylococcus aureus (up to 66% inhibition) whilst also enhancing osteogenesis (up to 3.6-fold increase in calcium deposition) and angiogenesis in vitro. Most significantly, when assessed in a chick embryo in vivo model, CuBG-CS not only demonstrated bio-compatibility, but also a significant angiogenic and osteogenic response, consistent with in vitro studies. Collectively, these results indicate that the CuBG-CS developed here show potential as a one-step osteomyelitis treatment: reducing infection, whilst enhancing bone healing.

Authors with CRIS profile

Anahi Philippart Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Francesca Ciraldo Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Royal College of Surgeons in Ireland (RCSI) IE Ireland (IE)

How to cite

APA:

Ryan, E.J., Ryan, A.J., Gonzalez-Vazquez, A., Philippart, A., Ciraldo, F., Hobbs, C.,... O'Brien, F.J. (2019). Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo. Biomaterials, 197, 405-416. https://dx.doi.org/10.1016/j.biomaterials.2019.01.031

MLA:

Ryan, Emily J., et al. "Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo." Biomaterials 197 (2019): 405-416.

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