Light-Responsive Size of Self-Assembled Spiropyran-Lysozyme Nanoparticles with Enzymatic Function

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Moldenhauer D, Fuenzalida Werner JP, Strassert CA, Gröhn F
Zeitschrift: Biomacromolecules
Jahr der Veröffentlichung: 2019
Band: 20
Heftnummer: 2
Seitenbereich: 979-991
ISSN: 1525-7797


Novel light-responsive nanoassemblies with switchable size and enzymatic activity are built from a protein and a water-soluble spiropyran. Assemblies are created by electrostatic self-assembly in aqueous solution such that the photochromic property of the spiropyran enables light responsiveness. Upon visible light exposure, the aggregate size increases from 200 to 400 nm. The enzyme retains its activity upon aggregation into the assembly, while it decreases through visible light irradiation. Fundamentally, we show how the two different spiropyran isomers, the open-ring merocyanine form and the closed-ring spiropyran form, bind differently to the protein, which triggers the assembly size and use of thermodynamic data to understand the binding process and the size response. Thus, as a proof of concept, a self-assembly driven light-tunable enzyme activity in conjunction with a triggerable assembly size is demonstrated for a model system. The concept bears future potential for various possible biological applications ranging from genetic control over vaccine applications to the detection of certain proteins.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Fuenzalida Werner, Juan Pablo Dr.
Interdisziplinäres Zentrum für Molekulare Materialien
Gröhn, Franziska Prof. Dr.
Professur für Molekulare Nanostrukturen
Moldenhauer, Daniel
Professur für Molekulare Nanostrukturen

Einrichtungen weiterer Autorinnen und Autoren

Westfälische Wilhelms-Universität (WWU) Münster


Moldenhauer, D., Fuenzalida Werner, J.P., Strassert, C.A., & Gröhn, F. (2019). Light-Responsive Size of Self-Assembled Spiropyran-Lysozyme Nanoparticles with Enzymatic Function. Biomacromolecules, 20(2), 979-991.

Moldenhauer, Daniel, et al. "Light-Responsive Size of Self-Assembled Spiropyran-Lysozyme Nanoparticles with Enzymatic Function." Biomacromolecules 20.2 (2019): 979-991.


Zuletzt aktualisiert 2019-06-03 um 16:38