Design of a Prediction Model for the Differentiation of Pasteurized Milk from Heated ESL Milk by Peptide Profiling.

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Details zur Publikation

Autorinnen und Autoren: Dalabasmaz S, Pischetsrieder M
Zeitschrift: Proteomics
Jahr der Veröffentlichung: 2019
Band: 19
Heftnummer: 7
Seitenbereich: e1800292
ISSN: 1615-9853


Abstract

This study
designed a prediction model to differentiate pasteurized milk from heated
extended shelf life (ESL) milk based on milk peptides. For this purpose,
quantitative peptide profiles of a training set of commercial samples including
pasteurized (n=20), pasteurized-ESL (n=13) and heated-ESL (n=16) milk were
recorded by MALDI-TOF-MS. Seven peptides were
selected as putative markers, and
cutoff levels and performance measures of each marker were defined by ROC analysis. The accuracy of these peptides in the
training set ranged between 71 and 90%. A prediction model was established based
on the combined cutoff levels and evaluated by an independent blind test set. The processing method of 19 out of 20
unknown milk samples was predicted correctly achieving 95% accuracy. Five peptides of the prediction model were
identified as αS1-casein182-199 (m/z 2014.0), αS1-casein180-199 (m/z 2216.1), αS1-casein1-24 (m/z 2910.6), β-casein108-125 (m/z 2126.0), and β-casein106-125 (m/z 2391.2) indicating thermal release and the action of
plasmin and cathepsins. Thus, the present study demonstrated that the milk
peptide profile reflects even minor differences in production parameters.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Dalabasmaz, Sevim Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Pischetsrieder, Monika Prof. Dr.


Zitierweisen

APA:
Dalabasmaz, S., & Pischetsrieder, M. (2019). Design of a Prediction Model for the Differentiation of Pasteurized Milk from Heated ESL Milk by Peptide Profiling. Proteomics, 19(7), e1800292. https://dx.doi.org/10.1002/pmic.201800292

MLA:
Dalabasmaz, Sevim, and Monika Pischetsrieder. "Design of a Prediction Model for the Differentiation of Pasteurized Milk from Heated ESL Milk by Peptide Profiling." Proteomics 19.7 (2019): e1800292.

BibTeX: 

Zuletzt aktualisiert 2019-08-04 um 14:53